Three new metabolites from the endophyte Fusarium proliferatum T2-10

One new cyclopeptide, cyclo-(L-Trp-L-Phe-L-Phe) (1), one new 2-pyridone derivative, fusarone A (3), and one new natural indole derivative, ethyl 3-indoleacetate (4), along with six known compounds were isolated from the endophytic fungus Fusarium proliferatum T2-10. The planar structures of three new compounds were identified by spectral methods including 1D and 2D NMR techniques, and the absolute configuration of compound 1 was elucidated by Marfey-MS method. In addition, all compounds were evaluated for their cytotoxic and antibacterial activities in vitro. Compound 2 showed remarkable cytotoxic activities against two human hepatoma cell lines SMMC7721 and HepG2 with IC50 values of 5.89 +/- 0.74 and 6.16 +/- 0.52 mu M, and showed moderate antibacterial activities against Staphylococcus aureus and Enterococcus faecalis with MIC values of 7.81 and 15.62 mu g/mL, respectively.

Automated summary

One new cyclopeptide, one new 2-pyridone derivative, and one new natural indole derivative, along with six known compounds, were isolated from the endophytic fungus Fusarium proliferatum T2-10. The structures of the new compounds were identified using spectral methods and the absolute configuration of compound 1 was determined using the Marfey-MS method. In addition, compound 2 exhibited significant cytotoxic and moderate antibacterial activities.