A novel exopolysaccharide from Weissella cibaria FAFU821: Structural characterization and cryoprotective activity

Exopolysaccharides produced by Weissella cibaria has attracted increasing attention owing to their biological activity. Here, a strain was isolated from the home-made fermented octopus, which was identified as W. cibaria FAFU821. In addition, the polysaccharide were isolated and purified by cellulose DE-52 column and Sephadex G-100 column, and named EPS821-1. In this work, the structure of EPS821-1 and its cryoprotective activity on Bifidobacterium longum subsp. longum F2 were investigated in vitro. These results suggested that the EPS821-1 is a novel glucan, which mainly consists of alpha-(1 -> 6) linkage with alpha-(1 -> 4), alpha-(1 -> 4,6) and alpha-(1 -> 3,6) residue as branches. In addition, EPS821-1 existed the three-dimensional network structure and exhibited the excellent cryoprotective activities for B. longum subsp. longum F2, which was 2.75 folds higher than that of the controls. This study provided scientific evidence and insights for the application of EPS821-1 as cryoprotection in food field.

Automated summary

The study investigates the structure and cryoprotective activity of the exopolysaccharide EPS821-1 produced by Weissella cibaria. EPS821-1 is found to have a novel glucan structure and exhibits excellent cryoprotective activities for Bifidobacterium longum subsp. longum F2. This research provides scientific evidence and insights for the potential application of EPS821-1 as a cryoprotectant in the food industry.