Split Intein: a Versatile Tool for Traceless Peptide Segment Ligation

Split intein can efficiently ligate peptide segments via a splicing reaction in a traceless manner and therefore has attracted great attention. Based on the structural characteristics and splicing reaction process of split intein, this paper comprehensively reviewed the recent progresses on the performance optimization and expanded applications of split intein, and revealed its great potential in the field of chemical protein synthesis as an increasingly sophisticated protein engineering technology. Finally, the challenges presented in the split inteinmediated protein trans-splicing and potential solutions in the future research were briefly discussed.

Automated summary

This paper comprehensively reviews the recent progress in performance optimization and expanded applications of split intein for peptide segment ligation. It highlights the great potential of split intein as a sophisticated protein engineering technology in the field of chemical protein synthesis. The challenges and potential solutions in split intein-mediated protein trans-splicing are also briefly discussed.