期刊
POULTRY SCIENCE
卷 102, 期 12, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.psj.2023.103098
关键词
Eimeria maxima; IFN-gamma inhibitory molecule; immune evasion; T cell subset
This study investigated the effects of Eimeria maxima IFN-gamma inhibitory molecules on the immune function of chicken peripheral blood mononuclear cells and T cell subsets. The results showed significant effects of these molecules on cell proliferation and cytokine production.
It has been reported that infection of chicken coccidian could inhibit the production of Th1 cytokine IFN-gamma, thereby evading clearance by the host immune system. The present study aimed to have a further investigation into the effects of Eimeria maxima IFN-gamma inhibitory molecules (EmHPSP-2 and EmHPSP-3) on the immune function of chicken peripheral blood mononuclear cells (PBMC) and various T cell subsets. First, separated PBMC or sorted T cell subsets were used for incubation with recombinant proteins of EmHPSP-2 (rEmHPSP-2) and EmHPSP-3 (rEmHPSP-3). Subsequently, the effects of rEmHPSP-2 and rEmHPSP-3 on proliferative capacity, nitric oxide (NO) release and mRNA levels of cytokines of the above cells were detected. The sorting purity of CD8(+), CD4(+) CD25(-), CD4(+), and CD4(+) CD25(+) T cells was 93.01, 88.88, 87.04, and 81.26%, respectively. The NO release of PBMC was significantly inhibited by rEmHPSP-2 and rEmHPSP-3. The proliferation of PBMC and CD4(+) T cells was significantly inhibited by rEmHPSP-2 and rEmHPSP-3, whereas CD8(+), CD4(+) CD25(-), and CD4(+) CD25(+) T cells was significantly promoted by the 2 proteins. The 2 proteins significantly downregulated interferon-gamma (IFN-gamma) mRNA level, upregulated the transcriptional levels of interleukin-10 (IL-10) and transforming growth factor-beta1 (TGF-beta 1) in PBMC. IFN-gamma and IL-2 transcriptional levels were markedly inhibited in CD8(+) T cells. IFN-gamma transcriptional level was significantly inhibited, but IL-4 was promoted by rEmHPSP-2 and rEmHPSP-3 in CD4(+) CD25(-) T cells. Meanwhile, the inhibitory effects of rEmHPSP-2 and rEmHPSP-3 on the transcriptional levels of IFN-gamma and IL-2 were more obvious in CD4(+) T cells containing CD25(+) cells compared with the CD25(+) cells depletion group. It was found that IL-10, TGF-beta 1, and cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) mRNA levels were significantly upregulated upon stimulation of chicken CD4(+) CD25(+) T cells by proteins. This study is not only of great significance to clarify the immune evasion mechanism of chicken coccidia, but also provides candidate antigen molecules for development of a novel vaccine against chicken coccidiosis.
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