期刊
MYCOTOXIN RESEARCH
卷 -, 期 -, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s12550-023-00509-y
关键词
Beauveria bassiana; Insecticide; Secondary metabolites; UPLC-Q-Orbitrap MS
Beauveria bassiana, an entomopathogenic fungus, produces eight insecticidal metabolites. A rapid analysis method using UPLC-Q-Orbitrap MS was developed to simultaneously detect and analyze these compounds. The main metabolites identified were bassianolide and beauvericin, indicating their potential as major toxins of B. bassiana.
Beauveria bassiana, a representative entomopathogenic fungus, is increasingly being utilized as an eco-friendly pest management alternative to chemical insecticides. This fungus produces a range of insecticidal secondary metabolites that act as antimicrobial and immunosuppressive agents. However, detailed qualitative and quantitative analysis related to these compounds remains scarce, we developed a method for the rapid analysis of these metabolites. Eight secondary metabolites (bassianin, bassianolide, beauvericin, beauveriolide I, enniatin A, A1, and B, and tenellin) were efficiently extracted when B. bassiana-infected Tenebrio molitor larvae were ground in 70% EtOH extraction solvent and subsequently subjected to ultrasonic treatment for 30 min. The eight metabolites were rapidly and simultaneously analyzed using ultra-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). Bassianolide (20.6-51.1 mu g/g) and beauvericin (63.6-109.8 mu g/g) were identified as the main metabolites in B. basssiana-infected larvae, indicating that they are likely major toxins of B. bassiana. Validation of the method exhibited recovery rates in the range of 80-115% and precision in the range of 0.1-8.0%, indicating no significant interference from compounds in the matrix. We developed a method to rapidly analyze eight insecticidal metabolites using UPLC-Q-Orbitrap MS. This can be extensively utilized for detecting and producing insecticidal fungal secondary metabolites.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据