Dual-responsive probe and DNA interstrand crosslink precursor target the unique redox status of cancer cells

Elevated GSH and H2O2 in cancer cells is sometimes doubted due to their contrary reactivities. Here, we construct a dual-responsive fluorescent probe to confirm the conclusion, and employ this to exploit a redox-inducible DNA interstrand crosslink (ICL) precursor. It crosslinks DNA upon activation by GSH and H2O2, affording an alternative dual-responsive strategy. Elevated GSH and H2O2 in some cancer cells is confirmed, and employed for the development of dual-responsive DNA ICL precursor.

Automated summary

In this study, a dual-responsive fluorescent probe was used to confirm the elevated levels of GSH and H2O2 in cancer cells. Based on these findings, a redox-inducible DNA interstrand crosslink precursor was developed.