期刊
MICROSCOPY RESEARCH AND TECHNIQUE
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1002/jemt.24457
关键词
cortical bone; intracortical vasculature; osteoporosis; synchrotron radiation x-ray imaging; trabecular bone
This study aimed to objectively diagnose osteoporosis (OP) by visualizing micro-damage caused by OP using synchrotron radiation-based mu CT (SR-mu CT). The results showed that the OVX group had smaller cortical bone volume and thickness, as well as lower trabecular bone volume/total volume compared to the SHAM group. In addition, the SHAM group had larger canal volume and lacuna volume than the OVX group.
Osteoporosis (OP) is difficult to diagnose through the three-dimensional visualization of micro-damage. In this study, aimed to make an objective diagnosis by visualizing micro-damage caused by OP using synchrotron radiation-based mu CT (SR-mu CT). Female mice (n = 12) were randomly divided into an ovariectomized group (OVX, n = 6) in which both ovaries were excised and OP occurred, and a sham-operated group (SHAM, n = 6). After six weeks, all femurs (left and right) were excised from both groups (n = 12 per group). Thereafter, femurs were randomly divided into SR-mu CT (OVX group, n = 6; SHAM group, n = 6) and mu CT (OVX group, n = 6; SHAM group, n = 6) groups. In the SR-mu CT group, micro-damage was visualized by manually segmenting the cortical bone, trabecular bone, and intracortical vasculature using a water-shedding algorithm. In addition, trabecular bone was obtained by automatic segmentation using mu CT. Cortical bone volume/total volume was greater (p = .015), and cortical thickness was greater in the SHAM group than in the OVX group (p = .007). Among the trabecular bone parameters, the bone volume/total volume (TV) in OVX was significantly lower than that in the SHAM group (p = .012). The canal volume was greater (p = .021) and lacuna volume was greater (p < .001) in the SHAM group than in the OVX group. We expect that it will be possible to analyze damage and recovery mechanisms in the field of rehabilitation. SR-mu CT has been proposed as an objective method for OP diagnosis as it allows the visualization of microstructures.
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