Assessment of Probiotic and Antioxidant Potential of Indigenous Lactobacillus Strains Isolated from Human Faecal Samples

This study aimed to isolate and characterize probiotic Lactobacilli from human faecal samples of Jammu region of India and evaluation of their antioxidative properties. A total of 29 Lactobacillus strains were isolated and tested for their ability to withstand different pH levels, high concentrations of bile salt and lysozyme along with their adhesion ability to different hydrocarbons and auto-aggregation. Selected probiotic Lactobacillus isolates were further examined for their antioxidant potential using ABTS, DPPH methods, and the ability to scavenge superoxide and hydroxyl radicals. The results showed that Lactobacillus LpJ1 (7.93 +/- 0.23) and LpJ5 (7.93 +/- 0.59) had the highest cell viability at a pH of 2.5, while Lactobacillus LpJ16 (7.91 +/- 0.48) had the highest resistance to bile salts. Many of the isolates also demonstrated good tolerance to lysozyme. The adhesion abilities of these isolates were characterized by cell surface hydrophobicity and auto aggregation which ranged between 50.32% to 77.8% and 51.02% to 78.95% respectively. In addition, Lactobacillus LpJ5 and LpJ8 showed excellent antioxidant activity. Based on these findings, the selected probiotic strains could be potential candidates for use in functional food to reduce oxidative stress.

Automated summary

This study isolated and characterized probiotic Lactobacilli from human fecal samples and evaluated their antioxidative properties. The findings showed that some Lactobacillus strains had the ability to withstand different environmental conditions and exhibited good antioxidant activity. These strains could potentially be used in functional foods to reduce oxidative stress.