期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1712, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.chroma.2023.464471
关键词
Cellulose monolith; Aqueous two-phase system; Bimodal porous structure; protein adsorption
This study proposes a novel method for preparing cellulose monoliths using an aqueous two-phase system without any organic solvents and surfactants. The obtained cellulose monolith exhibits a porous structure with a large surface area. Functionalized cellulose monoliths show potential as adsorbents.
Cellulose monolith has garnered significant interest in the field of biochromatography, which lies in its inter-connected porous structure, large surface area and biocompatibility. In this context, we propose a novel approach for preparing cellulose monoliths using an aqueous two-phase system devoid of any organic solvents and sur-factants. In this strategy, emulsifying cellulose solution into PEG 20,000 solution gives bicontinuous aqueous phases and further porous cellulose monolith after regeneration of dissolved cellulose. And the macroporous channels are derived from the removal of the PEG 20,000 aqueous phase while the micropores are from the phase separation of the cellulose phase. Physical characterizations reveal the obtained cellulose monolith exhibits exceptional column permeability of 1.36 x 10-11 m2 and a substantial surface area of 39.34 m2/g. Furthermore, cellulose monolith is functionalized with diethyl ethylamine hydrochloride (DEAE-HCl) to evaluate its potential as an anion adsorbent. Experimental results reveal that the DEAE-modified cellulose monolith possesses of adsorptive capacity of 316.58 mg/g of bovine serum albumin, along with fast adsorption kinetic. This study introduces an innovative strategy for fabricating porous cellulose monoliths tailored for biochromatography applications.
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