Assessment of visual function in blind mice and monkeys with subretinally implanted nanowire arrays as artificial photoreceptors

Retinal prostheses could restore image-forming vision in conditions of photoreceptor degeneration. However, contrast sensitivity and visual acuity are often insufficient. Here we report the performance, in mice and monkeys with induced photoreceptor degeneration, of subretinally implanted gold-nanoparticle-coated titania nanowire arrays providing a spatial resolution of 77.5 mu m and a temporal resolution of 3.92 Hz in ex vivo retinas (as determined by patch-clamp recording of retinal ganglion cells). In blind mice, the arrays allowed for the detection of drifting gratings and flashing objects at light-intensity thresholds of 15.70-18.09 mu W mm-2, and offered visual acuities of 0.3-0.4 cycles per degree, as determined by recordings of visually evoked potentials and optomotor-response tests. In monkeys, the arrays were stable for 54 weeks, allowed for the detection of a 10-mu W mm-2 beam of light (0.5 degrees in beam angle) in visually guided saccade experiments, and induced plastic changes in the primary visual cortex, as indicated by long-term in vivo calcium imaging. Nanomaterials as artificial photoreceptors may ameliorate visual deficits in patients with photoreceptor degeneration. Gold-nanoparticle-coated titania nanowire arrays subretinally implanted in blind mice and monkeys offer advantages in visual acuity and contrast sensitivity towards the restoration of visual function.

Automated summary

This study demonstrates the potential of subretinally implanted gold-nanoparticle-coated titania nanowire arrays to improve visual acuity and contrast sensitivity in mice and monkeys with induced photoreceptor degeneration, offering a promising approach for the restoration of visual function in patients with photoreceptor degeneration.