期刊
BMC MUSCULOSKELETAL DISORDERS
卷 24, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12891-023-07028-y
关键词
Erythropoietin; Apoptosis; Necrosis of the femoral head; STAT1-caspase 3
EPO can alleviate SANFH by inhibiting the STAT1-caspase 3 signaling pathway, suggesting its potential as a treatment option.
BackgroundSteroid-induced avascular necrosis of the femoral head (SANFH) is characterized by osteoblast apoptosis, leading to a loss of bone structure and impaired hip joint function. It has been demonstrated that erythropoietin (EPO) performs a number of biological roles.ObjectiveWe examined the effects of EPO on SANFH and its regulation of the STAT1-caspase 3 signaling pathway.MethodIn vitro, osteoblasts were treated with dexamethasone (Dex) or EPO. We identified the cytotoxicity of EPO by CCK-8, the protein expression of P-STAT1, cleaved-caspase9, cleaved-caspase3, Bcl-2, BAX, and cytochrome c by Western blotting, and evaluated the apoptosis of osteoblasts by flow cytometry. In vivo, we analyzed the protective effect of EPO against SANFH by hematoxylin and eosin (H&E), Immunohistochemical staining, and Micro-computed tomography (CT).ResultsIn vitro, EPO had no apparent toxic effect on osteoblasts. In Dex-stimulated cells, EPO therapy lowered the protein expression of BAX, cytochrome c, p-STAT1, cleaved-caspase9, and cleaved-caspase3 while increasing the expression of Bcl-2. EPO can alleviate the apoptosis induced by Dex. In vivo, EPO can lower the percentage of empty bone lacunae in SANFH rats.ConclusionThe present study shows that EPO conferred beneficial effects in rats with SANFH by inhibiting STAT1-caspase 3 signaling, suggesting that EPO may be developed as a treatment for SANFH.
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