Current status of β-lactam antibiotic use and characterization of β-lactam-resistant Escherichia coli from commercial farms by integrated broiler chicken operations in Korea

beta-Lactam antibiotics are one of the most clinical importance in human and veterinary medicine because they are used for both preventive and therapeutic purposes against several gram-positive, gram-negative, and anaerobic organisms. In this study, it was confirmed that beta-lactams (81.1%) were found to be significantly prescribed the most among 74 farms in 5 integrated broiler operations, and single prescription (84.6%), 2-day (41.5%) or 3-day (40.0%) administration, and 15 to 22 d of age (67.7%) administration was significantly higher in the farms (P < 0.05). Among the E. coli isolated from 74 farms in 5 integrated broiler operations, beta-lactam-resistant E. coli isolates were detected more frequently in fecal sample (94.6%) than in dust sample (60.8%) (P < 0.05). The prevalence of MDR in beta-lactam-resistant isolates, ranging from 88.1 to 96.5%, was significantly higher than that in non-beta-lactam-resistant isolates (P < 0.05), without significant differences among operations. Of 466 beta-lactam-resistant isolates, 432 (92.7%) isolates harbored beta-lactamase genes. The non-extended-spectrum beta-lactamase (ESBL) gene bla(TEM-1) (81.8%) showed the highest prevalence among isolates, followed by the non-ESBL gene bla(TEM-135) (6.4%) (P < 0.05). Five ESBL genes, SHV-12, OXA-1, CTX-M-27, CTX-M-55, and CTX-M-65, were found in 0.9 to 6.0% of the isolates. The pAmpC gene bla(CMY-2) was detected in 17 isolates (3.6%). These results suggest that feces and dust are important reservoirs of antimicrobial-resistant bacteria, highlighting the need to strengthen farm management regulations, such as cleaning, disinfection, and litter disposal and to reduce the use of antibiotics in broiler operations.

Automated summary

Beta-lactam antibiotics are widely used in human and veterinary medicine, but their excessive use has led to the spread of deadly antibiotic-resistant bacteria. This study found that beta-lactam antibiotics were commonly prescribed in integrated broiler operations, and their use was associated with an increase in beta-lactam-resistant E. coli isolates. These findings highlight the importance of strengthening farm management practices and reducing antibiotic use.