期刊
FOOD CHEMISTRY
卷 436, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2023.137714
关键词
Food allergy; Affinity peptide; Electrochemical modification; beta-lactoglobulin; Milk sample analysis
Harmful substances that cause food allergies present a significant threat to consumers and food safety. This study successfully developed a peptide-based sensor for detecting beta-lactoglobulin, a hazardous substance. The sensor exhibited a wide detection range and high recovery rates.
Harmful substances that cause food allergies can pose a significant threat to consumers along with food safety. According to the World Health Organization (WHO), approximately 10 % of the global population is currently affected by food allergies. Therefore, there is an urgent need for the development of more accurate and precise biosensors capable of detecting these hazardous substances including beta-lactoglobulin. Although numerous detection and analysis methods have been developed, they still suffer from various limitations. In this study, a tannic acid-polyethyleneimine (TA-PEI) network modified screen-printed electrodes (SPE) are newly developed and the binding sequence of peptide against beta-LG was successfully screened using random peptide library. A novel affinity peptide with the desired sequence of S-L-S-P-S-L-W-Q-V-S-M-L-G-G-G-G-E-P-L-Q-L-K-M against beta-lactoglobulin (beta-LG) is designed and synthesized. The synthesized affinity peptide was immobilized on TA-PEI modified SPE to develop peptide-based sensor against beta-LG for the first time. Under successful optimization, the developed sensor exhibited a linear relationship between 50 and 750 ng, with a Kd of 213.9 ng. In addition, the sensor was able to detect beta-LG in cow and goat milk, with average recoveries of 88.5 % and 92.2 %, respectively.
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