'Inert' co-formulants of a fungicide mediate acute effects on honey bee learning performance

Managed honey bees have experienced high rates of colony loss recently, with pesticide exposure as a major cause. While pesticides can be lethal at high doses, lower doses can produce sublethal effects, which may substantially weaken colonies. Impaired learning performance is a behavioral sublethal effect, and is often present in bees exposed to insecticides. However, the effects of other pesticides (such as fungicides) on honey bee learning are understudied, as are the effects of pesticide formulations versus active ingredients. Here, we investigated the effects of acute exposure to the fungicide formulation Pristine (active ingredients: 25.2% boscalid, 12.8% pyraclostrobin) on honey bee olfactory learning performance in the proboscis extension reflex (PER) assay. We also exposed a subset of bees to only the active ingredients to test which formulation component(s) were driving the learning effects. We found that the formulation produced negative effects on memory, but this effect was not present in bees fed only boscalid and pyraclostrobin. This suggests that the trade secret other ingredients in the formulation mediated the learning effects, either through exerting their own toxic effects or by increasing the toxicities of the active ingredients. These results show that pesticide co-formulants should not be assumed inert and should instead be included when assessing pesticide risks.

Automated summary

Managed honey bees have experienced high rates of colony loss due to pesticide exposure. Low doses of pesticides can weaken colonies, including impairing learning performance. The effects of fungicides on honey bee learning and the difference between pesticide formulations and active ingredients are understudied. This study found that acute exposure to the fungicide formulation Pristine had negative effects on honey bee memory, but these effects were not seen when bees were only exposed to the active ingredients. This suggests that other ingredients in the formulation mediated the learning effects. Pesticide co-formulants should be considered when assessing pesticide risks.