Bimodal modulation of in vitro angiogenesis with photoactive polymer nanoparticles

Angiogenesis is a fundamental process in biology, given the pivotal role played by blood vessels in providing oxygen and nutrients to tissues, thus ensuring cell survival. Moreover, it is critical in many life-threatening pathologies, like cancer and cardiovascular diseases. In this context, conventional treatments of pathological angiogenesis suffer from several limitations, including low bioavailability, limited spatial and temporal resolution, lack of specificity and possible side effects. Recently, innovative strategies have been explored to overcome these drawbacks based on the use of exogenous nano-sized materials and the treatment of the endothelial tissue with optical or electrical stimuli. Here, conjugated polymer-based nanoparticles are proposed as exogenous photo-actuators, thus combining the advantages offered by nanotechnology with those typical of optical stimulation. Light excitation can achieve high spatial and temporal resolution, while permitting minimal invasiveness. Interestingly, the possibility to either enhance (approximate to+30%) or reduce (up to -65%) the angiogenic capability of model endothelial cells is demonstrated, by employing different polymer beads, depending on the material type and the presence/absence of the light stimulus. In vitro results reported here represent a valuable proof of principle of the reliability and efficacy of the proposed approach and should be considered as a promising step towards a paradigm shift in therapeutic angiogenesis.

Automated summary

Angiogenesis is a critical process in biology, but conventional treatments for pathological angiogenesis have limitations. This study explores the use of nano-sized materials and optical or electrical stimuli to overcome these limitations. The use of conjugated polymer-based nanoparticles as photo-actuators shows promise in enhancing or reducing the angiogenic capability of endothelial cells. These findings provide valuable evidence for a potential paradigm shift in therapeutic angiogenesis.