☆ 3.8 Article

Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

APPLIED CATALYSIS B-ENVIRONMENT AND ENERGY (2024)

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Editorial Material Chemistry, Multidisciplinary

Comparative Performance of PETase as a Function of Reaction Conditions, Substrate Properties, and Product Accumulation

E. Erickson et al.

CHEMSUSCHEM (2021)

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Article Chemistry, Physical

Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy

Yinglu Cui et al.

Summary: This study successfully improved the robustness of PETase through a computational strategy, resulting in the redesign of a variant DuraPETase with significantly elevated melting temperature and enhanced degradation of PET films, as well as complete biodegradation of microplastics at mild temperatures. The design strategy presents opportunities for handling uncollectable PET waste and converting resulting monomers into high-value molecules.

ACS CATALYSIS (2021)

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Review Biotechnology & Applied Microbiology

Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects

Jiawei Liu et al.

Summary: This article discusses the global ranking and importance of polyurethanes, as well as their characteristics in terms of environmental factors and durability. It also highlights the need for proper disposal of polyurethane waste and the differences in polymer structure. Furthermore, it explores the discovery and application of polyurethane-degrading microbes and enzymes, as well as strategies for recycling and upcycling waste polymers.

BIOTECHNOLOGY ADVANCES (2021)

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Article Multidisciplinary Sciences

De novo protein design by deep network hallucination

Ivan Anishchenko et al.

Summary: Recent progress in protein structure prediction using deep neural networks has shown that these networks can be used to design new proteins with novel functions, by generating new folded proteins with sequences unrelated to those of naturally occurring proteins.

NATURE (2021)

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Article Biochemical Research Methods

Generating functional protein variants with variational autoencoders

Alex Hawkins-Hooker et al.

PLoS Computational Biology (2021)

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Review Microbiology

Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods

Maksim Sysoev et al.

Summary: Extremophiles are remarkable organisms that thrive in harsh environments and have developed strategies to overcome stress, making them valuable resources for biotechnology and biomedicine. However, due to difficulties in cultivation, only a small percentage of extremophiles have been discovered and characterized.

FRONTIERS IN MICROBIOLOGY (2021)

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Article Chemistry, Physical

The Current State of Research on PET Hydrolyzing Enzymes Available for Biorecycling

Fusako Kawai

Summary: This paper discusses the differences between thermophilic cutinases and mesophilic cutinases in PET hydrolysis. Thermophilic cutinases have a higher degradation ability in PET biorecycling due to their capability to work in high-temperature environments.

CATALYSTS (2021)

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Article Biochemistry & Molecular Biology

Analytical methods for the investigation of enzyme-catalyzed degradation of polyethylene terephthalate

Valentina Pirillo et al.

Summary: The study focuses on the persistent pollution concerns of PET plastic in global ecosystems and the enzymatic degradation research, including kinetic analysis of enzymes and methods study, providing important information for biologically recycling PET waste products.

FEBS JOURNAL (2021)

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Article Biochemistry & Molecular Biology

ANuPP: A Versatile Tool to Predict Aggregation Nucleating Regions in Peptides and Proteins

R. Prabakaran et al.

Summary: This study focuses on predicting aggregation-nucleating regions in peptides and proteins, developing an effective tool ANuPP. By analyzing atomic-level features, a better understanding of the aggregation mechanism is gained, providing important insights for protein aggregation research.

JOURNAL OF MOLECULAR BIOLOGY (2021)

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Article Multidisciplinary Sciences

Protein design and variant prediction using autoregressive generative models

Jung-Eun Shin et al.

Summary: The authors introduce a deep generative alignment-free model for sequence design applied to highly variable regions and design and test a diverse nanobody library with improved properties for selection experiments.

NATURE COMMUNICATIONS (2021)

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Article Chemistry, Medicinal

QM/MM Study of the Enzymatic Biodegradation Mechanism of Polyethylene Terephthalate

Sergio Boneta et al.

Summary: This study investigated the enzymatic biodegradation of PET at an atomic level using molecular dynamics simulations. Two hydrolases, Ideonella sakaiensis 201-F6 and leaf-branch compost cutinase, showed degradation activity towards PET. The study revealed a characteristic four-step degradation pathway for both enzymes, with LCC-ICCG variant outperforming PETase possibly due to its ability to work at higher temperatures and its intrinsic relationship with the crystallinity grade of the polymer.

JOURNAL OF CHEMICAL INFORMATION AND MODELING (2021)

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Article Biochemical Research Methods

A simple and novel method for the production of polyethylene terephthalate containing agar plates for the growth and detection of bacteria able to hydrolyze this plastic

Colin Charnock

Summary: Polyethylene terephthalate is commonly used in manufacturing various products, and there are known microbes capable of hydrolyzing this plastic, offering potential for effective waste management. Traditional screening methods involve cumbersome culture processes in the presence of film, while this article presents a rapid screening method based on agar plate culturing.

JOURNAL OF MICROBIOLOGICAL METHODS (2021)

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Article Multidisciplinary Sciences

Highly accurate protein structure prediction with AlphaFold

John Jumper et al.

Summary: Proteins are essential for life, and accurate prediction of their structures is a crucial research problem. Current experimental methods are time-consuming, highlighting the need for accurate computational approaches to address the gap in structural coverage. Despite recent progress, existing methods fall short of atomic accuracy in protein structure prediction.

NATURE (2021)

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Article Biochemistry & Molecular Biology

Assessment of the PETase conformational changes induced by poly(ethylene terephthalate) binding

Clauber Henrique Souza da Costa et al.

Summary: A bacterium strain with the ability to degrade PET was identified, and its enzyme IsPETase catalyzes the hydrolysis of PET. Molecular docking and dynamics simulations were used to analyze structural changes of IsPETase induced by PET binding, suggesting strategies for increasing the enzyme's thermal stability. This study provides insights into the molecular binding mechanism of PET into IsPETase structure and a computational strategy for mapping flexible regions of the enzyme.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2021)

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Article Multidisciplinary Sciences

Accurate prediction of protein structures and interactions using a three-track neural network

Minkyung Baek et al.

Summary: Through the three-track network, we achieved accuracies approaching those of DeepMind in CASP14, enabling rapid solution of challenging x-ray crystallography and cryo-electron microscopy structure modeling problems, and providing insights into the functions of proteins with currently unknown structure.

SCIENCE (2021)

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Article Environmental Sciences

Enzymatic degradation of polyethylene terephthalate nanoplastics analyzed in real time by isothermal titration calorimetry

Kristina Vogel et al.

Summary: Plastics waste poses adverse effects on ecosystems and human health when not properly managed. Current research focuses on microbial or enzymatic degradation of microplastics, while using isothermal titration calorimetry in combination with thermokinetic models for in-depth analysis of degradation processes. The new method allows quantification of enzymatic and microbial adsorption to nanoparticles in simulated aquatic conditions.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

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Article Chemistry, Physical

Techno-economic, life-cycle, and socioeconomic impact analysis of enzymatic recycling of poly(ethylene terephthalate)

Avantika Singh et al.

Summary: Esterases play a crucial role in enzymatic PET recycling, enabling efficient depolymerization to TPA and ethylene glycol, reducing energy consumption and greenhouse gas emissions, and generating more socio-economic benefits.

JOULE (2021)

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Review Chemistry, Multidisciplinary

Chemolytic depolymerisation of PET: a review

Elaine Barnard et al.

Summary: Developing new methods for plastic recycling is crucial for reducing CO2 emissions and improving resource efficiency. Chemical recycling, which involves depolymerization to reclaim original components, is gaining attention as a viable route. Lowering the energy barrier for depolymerization through catalysts under mild conditions is a key focus of research in this field.

GREEN CHEMISTRY (2021)

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Article Computer Science, Artificial Intelligence

Expanding functional protein sequence spaces using generative adversarial networks

Donatas Repecka et al.

Summary: De novo protein design for catalysis of any desired chemical reaction has long been a goal in protein engineering. ProteinGAN, a self-attention-based variant of generative adversarial networks, has been developed to learn natural protein sequence diversity and generate functional protein sequences. This AI approach shows potential in rapidly generating diverse functional proteins within biological constraints.

NATURE MACHINE INTELLIGENCE (2021)

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Article Food Science & Technology

Enhancement of Lipase CAL-A Selectivity by Protein Engineering for the Hydrolysis of Erucic Acid from Crambe Oil

Isabel Oro-Guinea et al.

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2020)

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Article Chemistry, Physical

Machine Learning in Enzyme Engineering

Stanislav Mazurenko et al.

ACS CATALYSIS (2020)

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Article Chemistry, Physical

Decomposition of the PET Film by MHETase Using Exo-PETase Function

Hye-Young Sagong et al.

ACS CATALYSIS (2020)

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Article Biochemical Research Methods

HECNet: a hierarchical approach to enzyme function classification using a Siamese Triplet Network

Safyan Aman Memon et al.

BIOINFORMATICS (2020)

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Review Biochemistry & Molecular Biology

Exploitation of Potential Extremophiles for Bioremediation of Xenobiotics Compounds: A Biotechnological Approach

Awadhesh Kumar Shukla et al.

CURRENT GENOMICS (2020)

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Article Microbiology

UV Pretreatment Impairs the Enzymatic Degradation of Polyethylene Terephthalate

Patricia Falkenstein et al.

FRONTIERS IN MICROBIOLOGY (2020)

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Article Multidisciplinary Sciences

An engineered PET depolymerase to break down and recycle plastic bottles

V. Tournier et al.

NATURE (2020)

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Article Biochemistry & Molecular Biology

EnzymeMiner: automated mining of soluble enzymes with diverse structures, catalytic properties and stabilities

Jiri Hon et al.

NUCLEIC ACIDS RESEARCH (2020)

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Article Biochemical Research Methods

A suspension-based assay and comparative detection methods for characterization of polyethylene terephthalate hydrolases

Jenny Arnling Baath et al.

ANALYTICAL BIOCHEMISTRY (2020)

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Article Multidisciplinary Sciences

Characterization and engineering of a two-enzyme system for plastics depolymerization

Brandon C. Knott et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

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Editorial Material Chemistry, Physical

Possibilities and limitations of biotechnological plastic degradation and recycling

Ren Wei et al.

NATURE CATALYSIS (2020)

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Article Biotechnology & Applied Microbiology

Gene amplification, laboratory evolution, and biosensor screening reveal MucK as a terephthalic acid transporter in Acinetobacter baylyi ADP1

Isabel Pardo et al.

METABOLIC ENGINEERING (2020)

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Article Cell Biology

Protein Solubility Predictions Using the CamSol Method in the Study of Protein Homeostasis

Pietro Sormanni et al.

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY (2019)

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Article Chemistry, Physical

Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation

Hyeoncheol Francis Son et al.

ACS CATALYSIS (2019)

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Article Chemistry, Multidisciplinary

Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures

Ren Wei et al.

ADVANCED SCIENCE (2019)

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Review Biotechnology & Applied Microbiology

Plastics: Environmental and Biotechnological Perspectives on Microbial Degradation

Dominik Danso et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2019)

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Article Environmental Sciences

Widespread distribution of PET and PC microplastics in dust in urban China and their estimated human exposure

Chunguang Liu et al.

ENVIRONMENT INTERNATIONAL (2019)

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Article Biochemical Research Methods

Protein-level assembly increases protein sequence recovery from metagenomic samples manyfold

Martin Steinegger et al.

NATURE METHODS (2019)

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Article Multidisciplinary Sciences

Deep learning enables high-quality and high-throughput prediction of enzyme commission numbers

Jae Yong Ryu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

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Article Multidisciplinary Sciences

Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate

Gottfried J. Palm et al.

NATURE COMMUNICATIONS (2019)

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Article Multidisciplinary Sciences

Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex

Diego F. Gauto et al.

NATURE COMMUNICATIONS (2019)

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Review Biochemistry & Molecular Biology

Toward understanding the origin and evolution of cellular organisms

Minoru Kanehisa

PROTEIN SCIENCE (2019)

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Article Multidisciplinary Sciences

Efficient Degradation of Poly(ethylene terephthalate) with Thermobifida fusca Cutinase Exhibiting Improved Catalytic Activity Generated using Mutagenesis and Additive-based Approaches

Makoto Furukawa et al.

SCIENTIFIC REPORTS (2019)

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Article Chemistry, Physical

A shared vision for macromolecular crystallography over the next five years

Andreas Forster et al.

STRUCTURAL DYNAMICS-US (2019)

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Article Biochemical Research Methods

Fast Turbidimetric Assay for Analyzing the Enzymatic Hydrolysis of Polyethylene Terephthalate Model Substrates

Matheus R. Belisario-Ferrari et al.

BIOTECHNOLOGY JOURNAL (2019)

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Article Multidisciplinary Sciences

Deciphering protein evolution and fitness landscapes with latent space models

Xinqiang Ding et al.

NATURE COMMUNICATIONS (2019)

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Editorial Material Multidisciplinary Sciences

Conformational fitting of a flexible oligomeric substrate does not explain the enzymatic PET degradation

Ren Wei et al.

NATURE COMMUNICATIONS (2019)

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Article Biochemical Research Methods

Determination of oligomers in virgin and recycled polyethylene terephthalate (PET) samples by UPLC-MS-QTOF

Sara Ubeda et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2018)

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Article Biophysics

Active Site Flexibility as a Hallmark for Efficient PET Degradation by I-sakaiensis PETase

Tobias Fecker et al.

BIOPHYSICAL JOURNAL (2018)

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Article Biochemistry & Molecular Biology

CavityPlus: a web server for protein cavity detection with pharmacophore modelling, allosteric site identification and covalent ligand binding ability prediction

Youjun Xu et al.

NUCLEIC ACIDS RESEARCH (2018)

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Article Biochemistry & Molecular Biology

SWISS-MODEL: homology modelling of protein structures and complexes

Andrew Waterhouse et al.

NUCLEIC ACIDS RESEARCH (2018)

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Article Multidisciplinary Sciences

Characterization and engineering of a plastic-degrading aromatic polyesterase

Harry P. Austin et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

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Article Multidisciplinary Sciences

Structural insight into molecular mechanism of poly (ethylene terephthalate) degradation

Seongjoon Joo et al.

NATURE COMMUNICATIONS (2018)

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Article Chemistry, Physical

Exploration of Enzyme Diversity by Integrating Bioinformatics with Expression Analysis and Biochemical Characterization

Pavel Vanacek et al.

ACS CATALYSIS (2018)

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Article Chemistry, Applied

Alteration of Chain Length Selectivity of Candida antarctica Lipase A by Semi-Rational Design for the Enrichment of Erucic and Gondoic Fatty Acids

Katja Zorn et al.

ADVANCED SYNTHESIS & CATALYSIS (2018)

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Article Biotechnology & Applied Microbiology

New Insights into the Function and Global Distribution of Polyethylene Terephthalate (PET)-Degrading Bacteria and Enzymes in Marine and Terrestrial Metagenomes

Dominik Danso et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2018)

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Article Biochemistry & Molecular Biology

Structural Dynamics of the PET-Degrading Cutinase-like Enzyme from Saccharomonospora viridis AHK190 in Substrate-Bound States Elucidates the Ca2+-Driven Catalytic Cycle

Nobutaka Numoto et al.

BIOCHEMISTRY (2018)

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Article Multidisciplinary Sciences

Structural investigation of a chaperonin in action reveals how nucleotide binding regulates the functional cycle

Guillaume Mas et al.

SCIENCE ADVANCES (2018)

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Article Chemistry, Multidisciplinary

Acceleration of Enzymatic Degradation of Poly(ethylene terephthalate) by Surface Coating with Anionic Surfactants

Makoto Furukawa et al.

CHEMSUSCHEM (2018)

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Article Chemistry, Multidisciplinary

Directed Evolution: Bringing New Chemistry to Life

Frances H. Arnold

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

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Article Biochemical Research Methods

DEEPre: sequence-based enzyme EC number prediction by deep learning

Yu Li et al.

BIOINFORMATICS (2018)

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Review Biochemistry & Molecular Biology

In-Vivo NMR Spectroscopy: A Powerful and Complimentary Tool for Understanding Environmental Toxicity

Monica Bastawrous et al.

METABOLITES (2018)

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Article Biochemistry & Molecular Biology

FireProt: web server for automated design of thermostable proteins

Milos Musil et al.

NUCLEIC ACIDS RESEARCH (2017)

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Article Biochemistry & Molecular Biology

Enzymatic recovery of polyester building blocks from polymer blends

Caroline Gamerith et al.

PROCESS BIOCHEMISTRY (2017)

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Article Biochemistry & Molecular Biology

Lipase from Candida antarctica (CALB) and cutinase from Humicola insolens act synergistically for PET hydrolysis to terephthalic acid

Adriano Carniel et al.

PROCESS BIOCHEMISTRY (2017)

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Article Engineering, Environmental

Mechanical and chemical recycling of solid plastic waste

Kim Ragaert et al.

WASTE MANAGEMENT (2017)

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Article Multidisciplinary Sciences

Structural insight into catalytic mechanism of PET hydrolase

Xu Han et al.

NATURE COMMUNICATIONS (2017)

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Article Multidisciplinary Sciences

Production, use, and fate of all plastics ever made

Roland Geyer et al.

SCIENCE ADVANCES (2017)

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Letter Biochemistry & Molecular Biology

Polyethylene bio-degradation by caterpillars of the wax moth Galleria mellonella

Paolo Bombelli et al.

CURRENT BIOLOGY (2017)

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Review Biochemistry & Molecular Biology

Ancestral protein reconstruction: techniques and applications

Rainer Merkl et al.

BIOLOGICAL CHEMISTRY (2016)

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Article Biotechnology & Applied Microbiology

Engineered Bacterial Polyester Hydrolases Efficiently Degrade Polyethylene Terephthalate Due to Relieved Product Inhibition

Ren Wei et al.

BIOTECHNOLOGY AND BIOENGINEERING (2016)

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Article Biochemistry & Molecular Biology

Progress in Protein Crystallography

Zbigniew Dauter et al.

PROTEIN AND PEPTIDE LETTERS (2016)

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Editorial Material Multidisciplinary Sciences

Feeding on plastic

Uwe T. Bornscheuer

SCIENCE (2016)

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Article Multidisciplinary Sciences

A bacterium that degrades and assimilates poly(ethylene terephthalate)

Shosuke Yoshida et al.

SCIENCE (2016)

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Article Biochemistry & Molecular Biology

Effect of Tris, MOPS, and phosphate buffers on the hydrolysis of polyethylene terephthalate films by polyester hydrolases

Juliane Schmidt et al.

FEBS OPEN BIO (2016)

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Article Biochemistry & Molecular Biology

A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate

Johannes Then et al.

FEBS OPEN BIO (2016)

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Article Biochemical Research Methods

Ca2+ and Mg2+ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca

Johannes Then et al.

BIOTECHNOLOGY JOURNAL (2015)

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Article Biochemistry & Molecular Biology

The TOPCONS web server for consensus prediction of membrane protein topology and signal peptides

Konstantinos D. Tsirigos et al.

NUCLEIC ACIDS RESEARCH (2015)

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Article Biochemical Research Methods

Ca2+ and Mg2+ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca

Johannes Then et al.

BIOTECHNOLOGY JOURNAL (2015)

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Article Biotechnology & Applied Microbiology

Structural basis for the Ca2+-enhanced thermostability and activity of PET-degrading cutinase-like enzyme from Saccharomonospora viridis AHK190

Takuya Miyakawa et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2015)

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Review Biotechnology & Applied Microbiology

Cold and hot extremozymes: industrial relevance and current trends

Felipe Sarmiento et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2015)

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Article Biochemistry & Molecular Biology

Crystal Structure and Thermodynamic and Kinetic Stability of Metagenome-Derived LC-Cutinase

Sintawee Sulaiman et al.

BIOCHEMISTRY (2014)

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Article Biochemical Research Methods

FISH Amyloid – a new method for finding amyloidogenic segments in proteins based on site specific co-occurence of aminoacids

Pawel Gasior et al.

BMC BIOINFORMATICS (2014)

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Article Biochemistry & Molecular Biology

The robustness and innovability of protein folds

Agnes Toth-Petroczy et al.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2014)

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Article Biochemistry & Molecular Biology

Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles

Ren Wei et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2014)

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Article Biotechnology & Applied Microbiology

Functional characterization and structural modeling of synthetic polyester-degrading hydrolases from Thermomonospora curvata

Ren Wei et al.

AMB EXPRESS (2014)

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Review Biotechnology & Applied Microbiology

Synthetic Polyester-Hydrolyzing Enzymes From Thermophilic Actinomycetes

Ren Wei et al.

ADVANCES IN APPLIED MICROBIOLOGY, VOL 89 (2014)

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Article Engineering, Chemical

Removal of Oligomers from Poly(ethylene terephthalate) Resins by Hydrothermal Extraction

Jun Inagaki et al.

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2013)

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Article Biochemistry & Molecular Biology

What Makes a Protein Fold Amenable to Functional Innovation? Fold Polarity and Stability Trade-offs

Eynat Dellus-Gur et al.

JOURNAL OF MOLECULAR BIOLOGY (2013)

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Editorial Material Multidisciplinary Sciences

Classify plastic waste as hazardous

Chelsea M. Rochman et al.

NATURE (2013)

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Article Biochemistry & Molecular Biology

Evolution of a designed retro-aldolase leads to complete active site remodeling

Lars Giger et al.

NATURE CHEMICAL BIOLOGY (2013)

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Review Polymer Science

Plastic Degradation and Its Environmental Implications with Special Reference to Poly(ethylene terephthalate)

Hayden K. Webb et al.

POLYMERS (2013)

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Article Biotechnology & Applied Microbiology

Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach

Sintawee Sulaiman et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2012)

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Article Biochemical Research Methods

A high-throughput assay for enzymatic polyester hydrolysis activity by fluorimetric detection

Ren Wei et al.

BIOTECHNOLOGY JOURNAL (2012)

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Article Biochemistry & Molecular Biology

PROSO II - a new method for protein solubility prediction

Pawel Smialowski et al.

FEBS JOURNAL (2012)

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Article Biochemical Research Methods

HHblits: lightning-fast iterative protein sequence searching by HMM-HMM alignment

Michael Remmert et al.

NATURE METHODS (2012)

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Article Biochemistry & Molecular Biology

IMG: the integrated microbial genomes database and comparative analysis system

Victor M. Markowitz et al.

NUCLEIC ACIDS RESEARCH (2012)

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Article Polymer Science

Enzymatic Surface Hydrolysis of PET: Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida

Enrique Herrero Acero et al.

MACROMOLECULES (2011)

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Article Biochemistry & Molecular Biology

HMMER web server: interactive sequence similarity searching

Robert D. Finn et al.

NUCLEIC ACIDS RESEARCH (2011)

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Article Chemistry, Multidisciplinary

Software News and Update AutoDock Vina: Improving the Speed and Accuracy of Docking with a New Scoring Function, Efficient Optimization, and Multithreading

Oleg Trott et al.

JOURNAL OF COMPUTATIONAL CHEMISTRY (2010)

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Article Multidisciplinary Sciences

Dynamic Regulation of Archaeal Proteasome Gate Opening As Studied by TROSY NMR

Tomasz L. Religa et al.

SCIENCE (2010)

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Article Biochemical Research Methods

QuickGO: a web-based tool for Gene Ontology searching

David Binns et al.

BIOINFORMATICS (2009)

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Article Polymer Science

Cutinase-Catalyzed Hydrolysis of Poly(ethylene terephthalate)

Asa M. Ronkvist et al.

MACROMOLECULES (2009)

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Article Multidisciplinary Sciences

De novo computational design of retro-aldol enzymes

Lin Jiang et al.

SCIENCE (2008)

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Article Agronomy

Alteration of lipase properties by protein engineering methods

Uwe T. Bornscheuer

OCL-OILSEEDS AND FATS CROPS AND LIPIDS (2008)

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Article Biochemical Research Methods

CTX-BLAST: context sensitive version of protein BLAST

Anna Gambin et al.

BIOINFORMATICS (2007)

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Article Biochemical Research Methods

AGGRESCAN:: a server for the prediction and evaluation of hot spots of aggregation in polypeptides

Oscar Conchillo-Sole et al.

BMC BIOINFORMATICS (2007)

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Article Multidisciplinary Sciences

Quantitative dynamics and binding studies of the 20S proteasome by NMR

Remco Sprangers et al.

NATURE (2007)

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Article Biochemistry & Molecular Biology

Biological degradation of synthetic polyesters - Enzymes as potential catalysts for polyester recycling

Rolf-Joachim Mueller

PROCESS BIOCHEMISTRY (2006)

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Article Biochemistry & Molecular Biology

CASTp: computed atlas of surface topography of proteins with structural and topographical mapping of functionally annotated residues

Joe Dundas et al.

NUCLEIC ACIDS RESEARCH (2006)

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Review Biotechnology & Applied Microbiology

Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems

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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2006)

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Article Biotechnology & Applied Microbiology

Enzymatic surface modification of poly(ethylene terephthalate)

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JOURNAL OF BIOTECHNOLOGY (2005)

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Article Polymer Science

Enzymatic degradation of poly(ethylene terephthalate):: Rapid hydrolyse using a hydrolase from T-fusca

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MACROMOLECULAR RAPID COMMUNICATIONS (2005)

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Article Biochemistry & Molecular Biology

The HHpred interactive server for protein homology detection and structure prediction

J Söding et al.

NUCLEIC ACIDS RESEARCH (2005)

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Article Biochemistry & Molecular Biology

ProFunc: a server for predicting protein function from 3D structure

RA Laskowski et al.

NUCLEIC ACIDS RESEARCH (2005)

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Article Polymer Science

Studies on the enzymatic hydrolysis of polyesters.: II.: Aliphatic-aromatic copolyesters

E Marten et al.

POLYMER DEGRADATION AND STABILITY (2005)

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Article Biochemistry & Molecular Biology

Combining prediction of secondary structure and solvent accessibility in proteins

R Adamczak et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2005)

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Review Biotechnology & Applied Microbiology

Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli -: art. no. 1

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MICROBIAL CELL FACTORIES (2005)

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Article Chemistry, Medicinal

Glide: A new approach for rapid, accurate docking and scoring. 1. Method and assessment of docking accuracy

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JOURNAL OF MEDICINAL CHEMISTRY (2004)

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Article Biochemistry & Molecular Biology

Biocatalytic modification of polyethylene terephthalate fibres by esterases from actinomycete isolates

M Alisch et al.

BIOCATALYSIS AND BIOTRANSFORMATION (2004)

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Article Biochemistry & Molecular Biology

Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel

R Chaloupková et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2003)

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Article Polymer Science

Glass transition and structural relaxation in semi-crystalline poly(ethylene terephthalate):: a DSC study

NM Alves et al.

POLYMER (2002)

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Article Chemistry, Analytical

Fe(II)-EDTA chelate-induced aromatic hydroxylation of terephthalate as a new method for the evaluation of hydroxyl radical-scavenging ability

XF Yang et al.

ANALYST (2001)

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The PSIPRED protein structure prediction server

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BIOINFORMATICS (2000)

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Article Biochemistry & Molecular Biology

KEGG: Kyoto Encyclopedia of Genes and Genomes

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NUCLEIC ACIDS RESEARCH (2000)

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