Spiky Artificial Peroxidases with V-O-Fe Pair Sites for Combating Antibiotic-Resistant Pathogens

With the sharp rise of antibiotic-resistant pathogens worldwide, it is of enormous importance to create new strategies for combating pathogenic bacteria. Here, we create an iron oxide-based spiky artificial peroxidase (POD) with V-O-Fe pair sites (V-Fe2O3) for combating methicillin-resistant Staphylococcus aureus (MRSA). The experimental studies and theoretical calculations demonstrate that the V-Fe2O3 can achieve the localized capture and killing bifunction from the spiky morphology and massive reactive oxygen species (ROS) production. The V-Fe2O3 can reach nearly 100 % bacterial inhibition over a long period by efficiently oxidizing the lipid membrane. Our wound disinfection results identify that the V-Fe2O3 can not only efficiently eliminate MRSA and their biofilm but also accelerate wound recovery without causing noticeable inflammation and toxicity. This work offers essential insights into the critical roles of V-O-Fe pair sites and localized capture and killing in biocatalytic disinfection and provides a promising pathway for the de novo design of efficient artificial peroxidases. An iron oxide-based spiky artificial peroxidase with V-O-Fe pair sites (V-Fe2O3) for efficient and selective ROS-catalysis has been reported. We demonstrate that the V-Fe2O3 can achieve the localized capture and killing bifunction from the spiky morphology and massive ROS (& sdot;OH and HClO) production, which efficiently eliminate MRSA and accelerate wound recovery without causing noticeable inflammation and toxicity.image

Automated summary

With the rise of antibiotic-resistant pathogens, new strategies for combating pathogenic bacteria are of great importance. In this study, an iron oxide-based spiky artificial peroxidase (POD) with V-O-Fe pair sites (V-Fe2O3) was created to combat methicillin-resistant Staphylococcus aureus (MRSA). Experimental studies and theoretical calculations demonstrated that the V-Fe2O3 can achieve localized capture and killing through its spiky morphology and production of massive reactive oxygen species (ROS). The V-Fe2O3 efficiently oxidized the lipid membrane, resulting in nearly 100% bacterial inhibition over a long period. Wound disinfection results showed that the V-Fe2O3 not only efficiently eliminated MRSA and their biofilm but also accelerated wound recovery without causing noticeable inflammation and toxicity.