Antioxidant peptides obtained from abalone muscle hot water extract during in-vitro simulated digestion and Caco-2 cell absorption

After abalone muscle hot water extract (AHE) was digested in-vitro, three components (F1, F2, and F3) were separated using the Sephadex G-15 column, and the antioxidant activity and absorption across Caco-2 cells were analysed. The result showed that the half-maximal inhibitory concentrations of F2 for scavenging hydroxyl and ABTS radicals were 0.763 and 0.028 mg mL(-1), respectively, which were lower than those of F1 and F3. Moreover, the Trolox equivalent antioxidant capacity was increased after the F2 was transported through the Caco-2 cell-monolayered Transwell chamber. The mass spectrometry analysis revealed that the transported samples contained oligopeptides (Leu-Tyr, Gly-Ala-Ala, and His-Gly-Ser-Ala), and the Leu-Tyr displayed the highest antioxidant activity and transport rate. Overall, these results suggest that oligopeptides with high antioxidant capacities can be produced from AHE through a combination of in-vitro digestion and absorption across Caco-2 cells.

Automated summary

This study successfully produced oligopeptides with high antioxidant capacities through in-vitro digestion and absorption across Caco-2 cells. Among the transported samples, Leu-Tyr oligopeptide displayed the highest antioxidant activity and transport rate, suggesting its potential as a functional food ingredient.