Multidimensional exploration of the bitterness amelioration effect of roasting on Wuyi Rock tea

The influence of roasting on tea bitterness remains unclear. With Wuyi Rock tea (WRT) as an example, this study investigated the impact of roasting on WRT's bitterness, utilizing an integrated approach involving sensory evaluation, bitter compound profiling, and cell-based calcium imaging. Sensory analysis revealed that roasting effectively reduced the perceived bitterness of WRT. This reduction was supported by decreases in various bitter compounds, including 19 flavanols, 11 flavonols, 12 phenolic acids, 2 purine alkaloids, and 9 bitter amino acids, which diminished by 16%, 26%, 19%, 2%, and 70%, respectively. Furthermore, we established two heterogeneous bitter receptor expression systems: TAS2R39/G alpha 15-HEK293T and TAS2R14/G alpha 15-HEK293T cell lines. These systems quantitatively confirmed the reduction in bitterness, demonstrating 51% and 62% decreases in intracellular calcium mobilization within the transfected cells, respectively. These findings provide compelling evidence for the bitterness-ameliorating effect of roasting, expanding our knowledge of the role of roasting in shaping the flavor of tea.

Automated summary

This study investigated the impact of roasting on the bitterness of Wuyi Rock tea (WRT) through an integrated approach. The results showed that roasting effectively reduced the perceived bitterness of WRT, supported by decreases in bitter compounds and calcium mobilization. These findings provide compelling evidence for the bitterness-ameliorating effect of roasting and expand our knowledge of the role of roasting in shaping tea flavor.