Integrating multi-index determination coupled with hierarchical cluster analysis to evaluate the quality consistency of PVE30, an anti-HSV glycoprotein macromolecule of Prunellae Spica

Article Polymer Science

Development and Validation of HPLC-DAD Method with Pre-Column PMP Derivatization for Monomeric Profile Analysis of Polysaccharides from Agro-Industrial Wastes

Aleksandra Vojvodic Cebin et al.

Summary: The study developed and validated an application for Zorbax Extend C18 columns to analyze neutral and acidic sugars in plant polysaccharides. The mobile-phase pH and acetonitrile content were found to be the most influential separation factors, and the method showed excellent linearity, repeatability, and intermediate precision. It was highly applicable for evaluating polysaccharides in selected wastes and had advantages in terms of accuracy in acidic sugar determination and simultaneous analysis with neutral sugars.

POLYMERS (2022)

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Article Food Science & Technology

Preparation, Antioxidant and Immunoregulatory Activities of a Macromolecular Glycoprotein from Salvia miltiorrhiza

Hai-Yu Ji et al.

Summary: In this study, the water-soluble glycoprotein from Salvia miltiorrhiza roots was successfully isolated and its structural properties, antioxidant and immunoregulatory activities were investigated. The glycoprotein showed strong scavenging effects on multiple free radicals and enhanced antioxidant enzyme activities and immunological indicators. It has the potential to be a novel edible functional compound in the food industry.

FOODS (2022)

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Article Biochemical Research Methods

Rapid analysis of phenyl isothiocyanate derivatives of amino acids present in Czech meads

Jitka Klikarova et al.

Summary: This study optimized a rapid analysis method for 21 amino acids in mead using reversed-phase high performance liquid chromatography with spectrophotometric detection. The total amino acid content in Czech meads was found to range from 134 to 828 mg/L, with both chromatographic and spectrophotometric methods providing overlapping results.

JOURNAL OF CHROMATOGRAPHY A (2021)

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Article Chemistry, Analytical

Multiple-fingerprint analysis of Poria cocos polysaccharide by HPLC combined with chemometrics methods

Yilin Zhang et al.

Summary: This study established multiple fingerprints to evaluate the quality of polysaccharides from Poria cocos (PCPs). Chemometric methods were used to analyze chromatographic data, distinguishing 16 batches of PCPs from different origins and identifying potential chemical markers for classification. The results revealed the presence of adulterants in two commercial samples.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2021)

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