期刊
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 4, 期 9, 页码 4842-4849出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.6b01124
关键词
Cellulose nanocrystals; Spacer; L-Leucine amino acid; Fluorescent; pH-Sensitive
资金
- National Natural Science Foundation of China [31370560]
- Natural Science Foundation of Fujian Province [2016J01088]
- Jiangsu Key Lab of Biomass-based Green Fuels [JSBGFC14016]
- open fund of Guangxi Key Laboratory of Chemistry and Engineering of Forest Products [GXFC15-04]
- Chemicals and Science Foundation for Distinguished Young Scholars of Fujian Agricultural and Forestry University [xjq201422]
Fluorescent labeled nanoparticles are expected to have potential applications in biological and imaging systems. In this study, in order to construct the fluorescent nanoparticles for pH-sensing, L-leucine amino acid is used as a spacer linker between cellulose nanocrystals (CNCs) and a pH-indicator dye (5 (and 6)-carboxy-2',7'-dichloro-fluorescein, CDCF). Characterization by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), and X-ray photoelectron spectroscopy (XPS) is adopted to follow the chemical modification, whereas the morphology is determined by atomic force microscopy (AFM). The crystallinity determined by NMR for the CNCs, A-CNCs, and F-A-CNCs is 78.32, 79.84, and 79.52, respectively. The quantitative analysis by elemental analysis revealed that the degree of cellulose hydroxyl group substitution (DS) of A-CNCs and F-A-CNCs is 0.05 and 0.27, respectively. According to the obtained results, CDCF fluorescein could be successfully labeled onto CNCs via L-leucine amino acid spacer linking, and the cellulose structure is stable during the labeling reaction. The fluorescent properties of the yielding pH-sensitive fluorescent CNCs (F-A-CNCs) are characterized by fluorescence spectrometer and imaged by confocal laser scanning microscopy (CLSM). The result shows that the fluorescence intensity of F-A-CNCs increases with increasing pH of the buffer from 2.28 to 10.84.
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