Physicochemical properties and antimicrobial activities of MytiLec-1, a member from the mytilectin family of mussels

MytiLec-1, the recombinant form of a mussel lectin from Mytillus galloprovincialis, was purified by affinity chromatography and showed the maximum hemagglutination activity at a temperature range of 10 degrees C to 40 degrees C and at pH 7.0 to 9.0. Denaturants like urea and acidic-guanidine inhibited its hemagglutination activity significantly. MytiLec-1 was found to be metal-independent though Ca2+ slightly increased the activity of chelated MytiLec-1. The lectin suppressed 65 % growth of Pseudomonas aeruginosa (ATCC 47085) at 200 mu g/ml and reduced the formation of biofilm (15 % at 200 mu g/ml). Comparing to Shigella sonnei (ATCC 29930), Shigella boydii (ATCC 231903) and Shigella dysenteriae (ATCC 238135), Bacillus cereus (ATCC 14579) was slightly more sensitive to MytiLec-1. At a concentration of 200 mu g/disc and 100 mu g/ml, MytiLec-1 prevented the growth of Aspergillus niger and agglutinated the spores of Aspergillus niger and Trichoderma reesei, respectively. Amino acid sequences, physicochemical properties and antimicrobial activities of MytiLec-1 were compared with three other lectins (CGL, MTL and MCL from Crenomytilus grayanus, Mytilus trossulas and Mytilus californianus, respectively) from the mytilectin family of bivalve mollusks. It reconfirms the function of these lectins to recognize pathogens and perform important roles in innate immune response of mussels.

Automated summary

MytiLec-1, a recombinant mussel lectin from Mytillus galloprovincialis, has been found to have antimicrobial activity by inhibiting the growth of Pseudomonas aeruginosa and preventing the formation of biofilms. It is also able to agglutinate the spores of Aspergillus niger and Trichoderma reesei. The study compares the amino acid sequences, physicochemical properties, and antimicrobial activities of MytiLec-1 with other lectins from bivalve mollusks, reaffirming the importance of lectins in the innate immune response of mussels.