期刊
BIOSENSORS & BIOELECTRONICS
卷 245, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2023.115828
关键词
Exosome phenotyping; MOF signal amplifier; Coordination self-assembly; High-affinity nanostar; Electrochemistry
This study utilizes the coordination between exosomes and metal-organic frameworks to develop an ultrasensitive detection method for tumor-derived exosomes. The method is highly specific and fast, allowing for the profiling of different cancer types and accurate diagnosis in a clinical setting. This strategy provides new opportunities for functional materials assembly and precision diagnostics.
The natural phospholipid structure imparts exosomes with not only cargo protection, but rich sites for coordination with metal-organic frameworks (MOFs) to assemble functional nanocomplexes, such as signal amplifiers. Here, we exploit exosomes to tune MOF signal amplifiers (Exo-MOF) for ultrasensitive phenotyping of tumorderived exosomes (tExo) based on self-driven coordination assembly and high-affinity nanostars. Exo-MOF leverages the specific coordination interaction between exosome and MOF that cages abundant redox molecules to assemble a super-redox signal amplifier. Moreover, the dispersed immuno-magnetic nanostars, which are assembled with antibodies on the surface of Au nanostars-coated magnetic nanoparticles, allow for rapid capturing of target tExo, addressing the limited mass transfer on electrode surface. Both Exo-MOF and highaffinity nanostars orchestrate the ultrahigh sensitivity (1 particle per 100 mu L, higher than that no Exo-MOF by at least 10-fold), specificity and speed of the sensor in tExo detection. Such a sensitive strategy allows profiling tExo across seven cancer types, and revealing the distinct exosomal surface expression patterns. Further, the ExoMOF sensor accurately distinguishes cancer patients from healthy individuals in a clinical cohort, and provides new opportunities for functional materials assembly and precision diagnostics.
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