期刊
ULTRASONICS
卷 137, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ultras.2023.107180
关键词
Sonodynamic inactivation; Conjugated polymer nanoparticles; Microbes; Biofilm; Drug-resistant microorganism
Sonodynamic inactivation (SDI) has an advantage over optical excitation-based protocols due to the deeper penetration of ultrasound (US) excitation. In this study, we applied conjugated polymer nanoparticles (CPNs) as an efficient sonosensitizer (SS) in SDI of pathogens and characterized the sonoreactor. We found that CPNs have a germicidal effect on planktonic cultures and mature biofilms, highlighting their potential for novel inactivation protocols.
Sonodynamic inactivation (SDI) of pathogens has an important advantage when compared to optical excitation-based protocols due to the deeper penetration of ultrasound (US) excitation in biological media or animal tissue. Sonosensitizers (SS) are compounds or systems that upon US stimulation in the therapeutic window (frequency = 0.8-3 MHz and intensity < 3 W/cm(2)) can induce damage to vital components of pathogenic microorganisms. Herein, we report the synthesis and application of conjugated polymer nanoparticles (CPNs) as an efficient SS in SDI of methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae and Candida tropicalis. A frequent problem in the design and testing of new SS for SDI is the lack of proper sonoreactor characterization which leads to reproducibility concerns. To address this issue, we performed dosimetry experiments in our setup. This enables the validation of our results by other researchers and facilitates meaningful comparisons with different SDI systems in future studies. On a different note, it is generally accepted that the mechanisms of action underlying SS-mediated SDI involve the production of reactive oxygen species (ROS). In an attempt to establish the nature of the cytotoxic species involved in our CPNs-based SDI protocol, we demonstrated that singlet oxygen (O-1(2)) does not play a major role in the observed sonoinduced killing effect. SDI experiments in planktonic cultures of optimally growing pathogens using CPNs result in a germicide effect on the studied pathogenic microorganisms. The implementation of SDI protocols using CPNs was further tested in mature biofilms of a MRSA resulting in-40 % reduction of biomass and similar to 70 % reduction of cellular viability. Overall, these results highlight the unique and unexplored capacity of CPNs to act as sonosensitizers opening new possibilities in the design and application of novel inactivation protocols against morbific microbes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据