Physiological responses and molecular mechanism of Chlorella sorokiniana to surgical mask exudates in wastewater

Microalgae-based bioremediation is likely to be challenged by the microplastics (MPs) in wastewater induced by the widely use of surgical masks (SMs) during COVID-19. However, such toxic impact was generally evaluated under high exposure concentrations of MPs, which was not in agreement with the actual wastewater environments. Therefore, this study investigated the microalgal cellular responses to the surgical mask exudates (SMEs) in wastewater and explored the underlying inhibitory mechanism from the molecular perspective. Specifically, 390 items/L SMEs (including 200 items/L MPs which was the actual MP level in wastewater) significantly inhibited nutrient uptake and photosynthetic activities interrupted peroxisome biogenesis and induced oxidative stress which destroyed the structure of cell membrane. Moreover, the SMEs exposure also affected carbon fixation pathways, suppressed ABC transporters while promoted oxidative phosphorylation processes for the ATP accumulation These comprehensive processes led to an 8.5% reduced microalgae growth and variations of cellular biocomponents including lipid, carbohydrate, and protein. The increased carotenoids and consumed unsaturated fatty acid were considered to alleviate the SMEs-induced stress, and the enhanced EPS secretion facilitated the homogeneous aggregation. These findings will enhance current understandings of the SMEs effects in wastewater on microalgae and further improve the practical relevance of microalgae wastewater bioremediation technology.

Automated summary

This study investigated the effects of surgical mask exudates (SMEs) in wastewater on microalgal cellular responses. The findings showed that SMEs significantly inhibited nutrient uptake and photosynthetic activities, disrupted peroxisome biogenesis, induced oxidative stress, and destroyed the structure of cell membrane. The exposure to SMEs also affected carbon fixation pathways, suppressed ABC transporters, and promoted oxidative phosphorylation processes. These findings contribute to our understanding of the effects of SMEs in wastewater on microalgae and improve the practical relevance of microalgae wastewater bioremediation technology.