期刊
BIOELECTROCHEMISTRY
卷 155, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2023.108589
关键词
N-CQDs; HER-2 biomarker; Immunosensor; Brest Cancer; Bio Sensor
In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
Using nitrogen-enhanced carbon quantum dots (N-CQDs) on a coated graphite sheet (GS) substrate (N-CQDs/ GS), a simple strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was investigated. The bovine serum albumin (BSA)-modified HER2 Antibody/N-CQDs/ GS immunoelectrode enabled excellent activity preservation for the biosensor, while the GS electrode provided a highly stable and conducting substrate. With a linear response range of 0.1 ng/mL-1 ng/mL and a low detection limit of 4.8 pg/mL. Meanwhile, the methodology demonstrated optimal specificity, stability, and reproducibility for detecting HER-2 protein in breast cancer patients untreated blood samples.
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