期刊
HELIYON
卷 9, 期 11, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.heliyon.2023.e21944
关键词
Soybean; Heat shock; Isoflavone; Polyphenol; Antioxidant; Anti-inflammatory; Anti -obesity
This study investigated the changes in functional components of soybeans under different temperature and duration conditions. The results showed that heat shock at 60 degrees C for 2 h increased polyphenols, antioxidants, and aglycon isoflavones in soybeans, resulting in increased anti-inflammatory and anti-obesity activity.
We know that heat shock can activate the functional components in soybeans, but we don't know the type, level, and duration of heat shock for maximum activation. To address this, the present study investigated the changes in functional components like polyphenols, antioxidants, and isoflavones in soybeans at various temperature levels and durations with their respective functionality or health benefits. For this, treated seed samples were extracted with 70 % ethanol. Heat shock at 60 degrees C for 2 h increased polyphenol content (60.67 % of control) and antioxidant activity for both ABTS (41.14 % of control) and DPPH (217.72 % of control). This also increased the beneficial aglycone form of isoflavones that includes daidzein (8.36-fold of control), glycitein (3.85-fold of control) and genistein (20.50-fold of control) but decreased the harmful beta-glucoside form (3.65-fold) including daiazin (1.84-fold of control); glycitin (1.45-fold of control) and genistin (23.88-fold of control) over untreated dry seed. This may happen because of the conversion of conjugated beta-glucoside isoflavones to their aglycone forms that have various health benefits. Maximum inhibition of NO production in RAW 264.7 cells was achieved by samples elicited for 2 h with 300 mu g/mL concentration. This sample also confirmed the maximum antiobesity activity treated against 3-T-3L1 cells. This study summarized that heat shock at 60 degrees C for 2 h increased polyphenols, antioxidants, and aglycon isoflavone in soybeans resulting in increased anti-inflammatory and anti-obesity activity.
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