Dynamic of Phenolic Compounds in Guadua angustifolia Kunth under Chemical, Organic, and Biological Fertilization

Guadua angustifolia produces phenolic compounds, and this production may be influenced by the application of chemical, organic, and biological fertilizers. Currently, the effect of such fertilizers on the synthesis dynamics of this group of metabolites in bamboo is unknown. In this study, the total phenolic content (TPC) and total flavonoid content (TFC) in the leaves of plants fertilized with diammonium phosphate (DAP) and humus in combination with the biofertilizers Promofort (R), Azospirillum brasilense, Pseudomonas fluorescens, and Stenotrophomonas sp. were determined using colorimetric techniques across three sampling events (four, five, and seven months after planting). Additionally, an approximation of the bacterial profile of G. angustifolia roots was performed using the DGGE-PCR fingerprint technique. Through repeated measures ANOVA (rmANOVA), it was determined that there is no statistically significant three-way interaction between humus or DAP application, biological fertilizers, and time for either TPC or TFC. However, there were interactions between the sampling event and the application of biological fertilizers for both TPC and TFC, with the latter being promoted by the application of Promofort (R). Finally, NMDS analyses and heatmaps with hierarchical clustering showed that the composition and abundance of OTUs in the bacterial profile varied with fertilization type and increased over time.

Automated summary

This study investigated the effects of chemical, organic, and biological fertilizers on the synthesis of phenolic compounds in Guadua angustifolia bamboo. It was found that the application of biological fertilizers promoted the increase in total phenolic content and total flavonoid content. Furthermore, the type of fertilizer also influenced the bacterial profile of the bamboo roots.