期刊
CHINESE JOURNAL OF NATURAL MEDICINES
卷 21, 期 10, 页码 730-744出版社
CHINESE JOURNAL NATURAL MEDICINES
DOI: 10.1016/S1875-5364(23)60466-2
关键词
Arthritis; Gut microbiota; Immunity; Inflammation; Tripterygium hypoglaucum
This study found that Tripterygium hypoglaucum extract can protect against rheumatoid arthritis by manipulating the gut microbiota and regulating the TLR4/ MyD88/ MAPK signaling pathway. Additionally, treatment with Tripterygium hypoglaucum extract failed to improve arthritis symptoms and signaling pathways in pseudo germ-free mice, further validating the importance of the gut microbiota.
Traditionally, Tripterygium hypoglaucum (Levl.) Hutch (THH) are widely used in Chinese folk to treat rheumatoid arthritis (RA). This study aimed to investigate whether the anti-RA effect of THH is related with the gut microbiota. The main com-ponents of prepared THH extract were identified by HPLC-MS. C57BL/6 mice with adjuvant-induced arthritis (AIA) were treated with THH extract by gavage for one month. THH extract significantly alleviated swollen ankle, joint cavity exudation, and articular cartil-age destruction in AIA mice. The mRNA and protein levels of inflammatory mediators in muscles and plasma indicated that THH ex-tract attenuated inflammatory responses in the joint by blocking TLR4/MyD88/MAPK signaling pathways. THH extract remarkably restored the dysbiosis of the gut microbiota in AIA mice, featuring the increases of Bifidobacterium, Akkermansia, and Lactobacillus and the decreases of Butyricimonas, Parabacteroides, and Anaeroplasma. Furthermore, the altered bacteria were closely correlated with physiological indices and drove metabolic changes of the intestinal microbiota. In addition, antibiotic-induced pseudo germ-free mice were employed to verify the role of the intestinal flora. Strikingly, THH treatment failed to ameliorate the arthritis symptoms and signaling pathways in pseudo germ-free mice, which validates the indispensable role of the intestinal flora. For the first time, we demonstrated that THH extract protects joint inflammation by manipulating the intestinal flora and regulating the TLR4/MyD88/MAPK signaling pathway. Therefore, THH extract may serve as a microbial modulator to recover RA in clincial prac-tice.ver RA in clincial practice.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据