Butin prevent liver damage triggered by D-galactosamine via regulating lipid peroxidation and proinflammatory cytokines in rodents

Objectives: Liver damage is becoming a more severe problem around the world. Unfortunately, there is still a great need for clinically effective pharmacological therapy for liver injury. The investigation aimed to assess the favorable outcome of butin hepatoprotective efficacy against D-galactosamine (D-GalN)-produced liver damage in experimental rats.Methods: A single dose of D-GalN 400 mg/kg b. wt. was injected intraperitoneally, 24 hr prior to scarifi-cation of rats to cause liver damage. The liver injury was assessed biochemically, investigating parame-ters like aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT), c- glutamyl transferase (GGT), total-bilirubin,-albumin and-protein. Butin (25 and 50 mg/kg b. wt.) was managed for 21st days and at the end of the experimental period, normal control, D-GalN control, and butin treatment group rats for further biochemical as well as histopathological consideration.Results: Butin pretreatment significantly attenuated D-GalN-induced hepatocytes damage by reducing hepatic markers and its ability in attenuating oxidative damage (MDA, GSH, SOD, CAT) and pro-inflammatory cytokine (TNF-a, IL-6, IL-1b) and MPO. Histopathological assessment of the liver and bio-chemical estimation confirmed the hepatoprotective effects of butin. Conclusions: These results demon-strated that butin protects experimental rats against D-GalN-induced liver injury by modulating lipid peroxidation and pro-inflammatory cytokine. Current research will give preliminary experimental evi-dence for butin, and possible therapeutic effects in liver disorders.(c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

This study aimed to evaluate the therapeutic effects of butin on D-GalN-induced liver damage. The experimental results demonstrated that butin had a hepatoprotective effect on rats by reducing liver cell damage, oxidative damage, and pro-inflammatory cytokine levels.