Systematic analysis of Prx genes in the Brachypodium genus and their expression pattern under abiotic constraints

Peroxiredoxins (Prx) are ubiquitous peroxidases required for the removal of excess free radicals produced under stress conditions.Peroxiredoxin genes (Prx) in the Brachypodium genus were identified using bioinformatics tools and their expression profiles were determined under abiotic stress using RT-qPCR. The promoter regions of Prx genes contain several cis-acting elements related to stress response. In silico expression analysis showed that B. distachyon Prx genes (BdPrx) are tissue specific. RT-qPCR analysis revealed their differential expression when exposed to salt or PEG-induced dehydration stress. In addition, the upregulation of BdPrx genes was accompanied by accumulation of H2O2. Exogenous application of H2O2 induced expression of almost all BdPrx genes. The identified molecular interaction network indicated that Prx proteins may contribute to abiotic stress tolerance by regulating key enzymes involved in lignin biosynthesis. Overall, our findings suggest the potential role of Prx genes in abiotic stress tolerance and lay the foundation for future functional analyses aiming to engineer genetically improved cereal lines.

Automated summary

The study identified Prx genes in the Brachypodium genus and examined their expression profiles under abiotic stress. The results showed that these genes are tissue specific and differentially expressed under salt or dehydration stress, accompanied by H2O2 accumulation. The study also revealed that Prx proteins may regulate key enzymes involved in lignin biosynthesis. These findings contribute to the understanding of the potential role of Prx genes in abiotic stress tolerance and provide a foundation for future research on genetically improving cereal lines.