期刊
FRONTIERS IN MICROBIOLOGY
卷 14, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2023.1267237
关键词
Streptococcus thermophilus; sugar transporter; galactose utilization; lactose permease; lactose utilization
类别
S. thermophilus strains have strain-specific utilization of galactose, and multiple genetic factors can affect their sugar utilization phenotype.
Introduction Streptococcus thermophilus is a traditional starter for dairy products. The lactose rich in milk is the main carbon source for the growth of S. thermophilus. However, the utilization of galactose by S. thermophilus is strain-specific, and many genetic factors can affect the sugar utilization phenotype of S. thermophilus strains.Methods In this study, S. thermophilus A25, which is capable of utilizing lactose and galactose, was used as the starting strain to construct lactose permease-deficient mutant S. thermophilus Delta lacS. Subsequently, the complement vectors expressing complete lactose permease of S. thermophilus and its N-terminal 1-486 amino acid residues were constructed and transformed into S. thermophilus Delta lacS, respectively. Meanwhile, complement vectors expressing lactose permease and galactose/proton symporter of Escherichia coli were also constructed.Results and Discussion Results showed that S. thermophilus Delta lacS lost the ability to utilize lactose and galactose. By measuring the growth of the recombinant strains, it was found that the strain expressing complete lactose permease of S. thermophilus recovered the growth ability in lactose and galactose medium, while the strain expressing N-terminal of lactose permease recovered the growth ability only in lactose medium. Furthermore, the transformation of S. thermophilus Delta lacS was not successful with the complement vector expressing E. coli lactose permease, while the strain expressing E. coli galactose/proton symporter could recover its growth ability in the galactose medium. These results suggest that the properties of sugar transporters play an important role in galactose utilization by S. thermophilus.
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