An ultrasound-controllable ROS-responsive nanoplatform for O2 and NO generation to enhance sonodynamic therapy against multidrug-resistant bacterial infections

Antimicrobial sonodynamic therapy (SDT) has broad application potential in the eradication of multidrug-resistant (MDR) bacterial infections due to its non-invasiveness, absence of resistance concern, and high cytotoxicity. However, the hypoxic infection microenvironment and the rapid depletion of O-2 during SDT severely limit the therapeutic efficacy of SDT. Herein, an ultrasound-controllable ROS-responsive nanoplatform (FOT/Fe3O4@Lipo-ICG) was constructed and prepared by encapsulating FOT and Fe3O4 nanoparticles (Fe3O4 NPs) within sonosensitiser ICG-modified liposomes. Both in vitro and in vivo, we observed that ICG conjugation on the surface of liposomes could effectively maintain good dispersion and prevent ICG aggregates in complex biological matrices. In addition, liposomes could significantly block the catalytic activity of Fe3O4 NPs, as well as the release of FOT, whereas upon US irradiation, the catalytic activity of Fe3O4 NPs was recovered to catalyse the decomposition of endogenous H2O2 into O-2 and OH. Meanwhile, the FOT was successfully released to react with endogenous glutathione to sequentially produce NO. Based on the aforementioned advantages, the FOT/Fe3O4@Lipo-ICG demonstrated potent efficacy in eradicating methicillin-resistant Staphylococcus aureus-induced local infection and sepsis resulting from local infection. Thus, the developed US-controllable nanoplatform offers a promising strategy for enhancing SDT for eradicating MDR bacterial infections.

Automated summary

This study developed an ultrasound-controllable nanoplatform to enhance antimicrobial therapy. By encapsulating FOT and Fe3O4 nanoparticles within ICG-modified liposomes, the control over the infection microenvironment and rapid O-2 depletion during therapy was achieved. The results showed the potential of this platform in eradicating MDR bacterial infections.