期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 145, 期 47, 页码 25776-25788出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.3c09335
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This study presents a single-molecule approach using solid-state nanopores and multiplexed DNA barcoding for the detection and quantification of protein oligomers. By studying alpha-synuclein oligomers and inhibitors of alpha-synuclein aggregation, the potential applicability of this method to the development of diagnostic and therapeutic methods for Parkinson's disease is demonstrated.
Misfolded protein oligomers are of central importance in both the diagnosis and treatment of Alzheimer's and Parkinson's diseases. However, accurate high-throughput methods to detect and quantify oligomer populations are still needed. We present here a single-molecule approach for the detection and quantification of oligomeric species. The approach is based on the use of solid-state nanopores and multiplexed DNA barcoding to identify and characterize oligomers from multiple samples. We study alpha-synuclein oligomers in the presence of several small-molecule inhibitors of alpha-synuclein aggregation as an illustration of the potential applicability of this method to the development of diagnostic and therapeutic methods for Parkinson's disease.
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