Electroanalytical Investigation of 2-(2-Methylbenzyl)-4(7)-phenyl-1H-benzo[d]imidazole, a Novel Benzimidazole Derivative

Electrochemical analysis of 2-(2-methylbenzyl)-4(7)-phenyl-1-H-benzo[d]imidazole (MPB), a new benzimidazole derivative with tyrosinase inhibitory effect and antioxidant activity, was performed in this study. The electro-oxidation behavior of MPB was investigated by cyclic voltammetry (CV), differential pulse stripping voltammetry (DPSV), and square wave voltammetry (SWV) on glassy carbon electrode. The oxidation process of MPB showed an irreversible behavior. The highest peak current was obtained in 0.5 M H2SO4 containing 10 % methanol and used as the supporting electrolyte. As a result of the calibration study, two linear responses in the range of 0.06-1.00 mu M and 2.00-100 mu M were obtained with a detection limit of 10.4 nM by DPSV. Linearity for SWV was between 0.1 and 10.0 mu M with a detection limit of 29.3 nM. Precision of the methods was obtained as relative standard deviation (RSD) and was below 2.0 %. Developed methods were applied for the recovery of MPB from spiked serum samples. Accordingly, recoveries of 100.39 % for DPSV and 100.07 % for SWV were obtained with RSD below 0.48 %. MPB was stable for at least 2 weeks as a result of retaining 97 % of the peak current when stored at +4 degrees C. Finally, the possible responsible group in MPB oxidation was proposed.

Automated summary

Electrochemical analysis of a new benzimidazole derivative, 2-(2-methylbenzyl)-4(7)-phenyl-1-H-benzo[d]imidazole (MPB), was performed to study its tyrosinase inhibitory effect and antioxidant activity. The oxidation behavior of MPB was investigated using cyclic voltammetry, differential pulse stripping voltammetry, and square wave voltammetry, which revealed its irreversible nature. Calibration experiments showed that DPSV and SWV methods could detect MPB in different concentration ranges, with low detection limits and good precision.