期刊
POLYMERS
卷 8, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/polym8080280
关键词
lignin; biocatalyzed polymerization; enzyme immobilization; laccase; green processing
资金
- PhD School DokIn'Holz - Austrian Federal Ministry of Science, Research and Economy
- Austrian Centre of Industrial Biotechnology
- Austrian Research Promotion Agency (FFG)
Enzymatic polymerization of lignin is an environmentally-friendly and sustainable method that is investigated for its potential in opening-up new applications of one of the most abundant biopolymers on our planet. In this work, the laccase from Myceliophthora thermophila was successfully immobilized onto Accurel MP1000 beads (67% of protein bound to the polymeric carrier) and the biocatalyzed oxidation of Kraft lignin (KL) and lignosulfonate (LS) were carried out. Fluorescence intensity determination, phenol content analysis and size exclusion chromatography were performed in order to elucidate the extent of the polymerization reaction. The collected results show an 8.5-fold decrease of the LS samples' fluorescence intensity after laccase-mediated oxidation and a 12-fold increase of the weight average molecular weight was obtained.
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