Essential gene complement of Planctopirus limnophila from the bacterial phylum Planctomycetes

Planctopirus limnophila belongs to the bacterial phylum Planctomycetes, a relatively understudied lineage with remarkable cell biology features. Here, we report a genome-wide analysis of essential gene content in P. limnophila. We show that certain genes involved in peptidoglycan synthesis or cell division, which are essential in most other studied bacteria, are not essential for growth under laboratory conditions in this species. We identify essential genes likely involved in lipopolysaccharide biosynthesis, consistent with the view of Planctomycetes as diderm bacteria, and highlight other essential genes of unknown functions. Furthermore, we explore potential stages of evolution of the essential gene repertoire in Planctomycetes and the related phyla Verrucomicrobia and Chlamydiae. Our results provide insights into the divergent molecular and cellular biology of Planctomycetes.

Automated summary

This study presents a genome-wide analysis of essential gene content in Planctopirus limnophila. The findings indicate that certain genes involved in peptidoglycan synthesis or cell division, which are essential in most bacteria, are not essential for growth under laboratory conditions in this species. Additionally, essential genes likely involved in lipopolysaccharide biosynthesis are identified, supporting the view of Planctomycetes as diderm bacteria. The study also explores the evolutionary stages of essential gene repertoire in Planctomycetes and related phyla.