期刊
FOOD CHEMISTRY
卷 437, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2023.137898
关键词
O-alpha-glycoligase; Transglycosylation; Enzyme engineering; Genistein alpha-diglucoside; Water solubility
This study successfully improved the synthesis efficiency of Genistein through enzyme mutation, and found that the synthesized α-diglucoside had better water solubility and bioavailability compared to Genistein itself.
Genistein is one of isoflavones, showing various biological functions for human health. MalA-D416A, termed O-alpha-glycoligase, is an acid/base catalytic residue-deficient mutant of a alpha-glucosidase from Sulfolobus solfataricus, synthesizing genistein 7-O-alpha-glucoside using alpha-glucosyl fluoride as the donor substrate. Through mutagenesis toward MalA-D416A, an O-alpha-glycoligase variant with two mutations (D416R and Q450S) was identified as a biocatalyst with a 58.8-fold enhanced catalytic efficiency for genistein compared to the parent enzyme. The use of a 2:1 ratio of alpha-glucosyl fluoride and genistein at pH 9 facilitated the synthesis of genistein 7,4'-O-alpha-diglucoside by MalA-D416R/Q450S. The alpha-diglucoside exhibited 2,459-fold improved water solubility compared to genistein itself as well as facile deglycosylation by the intestinal alpha-glucosidase from rat, suggesting the potential of the alpha-diglucoside for improved bioavailability in human intestine. Through molecular docking analyses the modulation of the active site conformation by these mutations was expected for proper binding of both genistein and the monoglucoside.
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