4.7 Article

CRX haploinsufficiency compromises photoreceptor precursor translocation and differentiation in human retinal organoids

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STEM CELL RESEARCH & THERAPY
卷 14, 期 1, 页码 -

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BMC
DOI: 10.1186/s13287-023-03590-3

关键词

CRX; Haploinsufficiency; Photoreceptor; Human; Retinal organoids; Development; Inherited; Mutation; Disease

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We created monoallelic CRX by replacing one allele with tdTomato in human embryonic stem cells (hESCs) and studied the pathogenesis in hESCs-derived retinal organoids. We found that gene haploinsufficiency is the mechanism for the dominant pathogenicity of CRX, and observed the translocation of monoallelic CRX+ cells in early retinal differentiation.
BackgroundThe CRX-associated autosomal dominant retinopathies suggest a possible pathogenic mechanism of gene haploinsufficiency. However, based on reported human patient cases and studies with mouse models, it is hard to confirm the specific weight of haploinsufficiency in pathogenesis due to the interspecies gaps between gene expression and function.MethodsWe created monoallelic CRX by replacing one allele with tdTomato in human embryonic stem cells (hESCs) and subsequently dissect pathogenesis in hESCs-derived retinal organoids. We used transcriptome and immunofluorescence analyses to dissect phenotypic differences between CRX-monoallelic knockout and control wildtype organoids. For location analysis of CRX+ cells, a CRX-expression-tracing system was constructed in control hESCs. We implemented long-term live-cell imaging to describe the translocation of CRX+ cells between two groups in early organoid differentiation. The expression pattern of these dynamic differences was validated using RNA-seq and immunofluorescence assays.ResultsWe identified delayed differentiation of outer nuclear layer (ONL) stratification along with thinner ONL, serious loss of photoreceptor outer segments, as well as downregulated expression of gene for phototransduction and inner/outer segment formation. By live-cell imaging and immunostaining, we observed the overtension of actomyosin network and the arrested translocation of monoallelic CRX+ cells in the early stage of retinal differentiation.ConclusionsWe confirmed that gene haploinsufficiency is the mechanism for the dominant pathogenicity of CRX and discovered that CRX regulated postmitotic photoreceptor precursor translocation in addition to its specification of photoreceptor cell fates during human retinal development. These findings revealed a new underlying mechanism of CRX dominant pathogenesis and provided a new clue for the treatment of CRX-associated human retinopathies.

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