The associations of erythrocyte membrane polyunsaturated fatty acids with skeletal muscle loss: A prospective cohort study

Background & aims: Polyunsaturated fatty acids (PUFAs) may play a vital role in maintaining skeletal muscle mass in the aged population. This study investigated the longitudinal relationship between the concentrations of erythrocyte membrane PUFAs and age-related changes in skeletal muscle mass over an average 6.5 years of follow-up in a Chinese middle-aged and older adult population.Methods: A total of 1494 participants aged 57.4 +/- 4.7 years were included in this study. Skeletal muscle mass was determined using dual-energy X-ray absorptiometry. Per year percent changes in the skeletal muscle index (Delta% SMI), appendicular skeletal muscle index (Delta% ASMI), and total body lean mass index (Delta% TBLMI) from baseline were calculated. Concentrations of total and individual cis-n-3 and cis-n-6 PUFAs of the erythrocyte membrane were determined using gas-liquid chromatography.Results: Fully adjusted linear regression models showed that per unit increases in the concentrations of C18:2 n-6, C20:4 n-6, C22:4 n-6, and total n-6 PUFAs resulted in increases of 0.022%-0.155 % in the Delta% SMI (P for linearity: <0.001-0.006). Restricted cubic spline analysis revealed an inverted U-shaped relationship between the concentrations of C20:2 n-6, C22:5 n-3, C22:6 n-3, and total n-3 PUFAs and the Delta% SMI (P for non-linearity: <0.001-0.036). In addition, an inverted U-shaped curve was also detected for the relationships of the linoleic acid/alpha-linolenic acid ratio (P for non-linearity = 0.010) and n-6/n-3 PUFA ratio (P for non-linearity = 0.013) with the Delta% SMI, with the Delta% SMI peaking at respective ratios of 124.96 and 3.69. Similar associations were revealed by the Bayesian kernel machine regression model. No interaction effect was detected between the individual PUFAs for the Delta% SMI in the bivariate exposure-response analysis. Overall, similar results were observed for the Delta% ASMI and Delta% TBLMI.Conclusions: The associations between different individual PUFAs and age-related muscle loss in middle-aged and older adults may be different. Our results suggest that high concentrations of erythrocyte membrane n-6 PUFAs may be correlated with less skeletal muscle mass loss, whereas extremely high concentrations of n-3 PUFAs may be correlated with more muscle loss.

Automated summary

This study investigated the longitudinal relationship between erythrocyte membrane PUFAs concentrations and age-related changes in skeletal muscle mass in a Chinese middle-aged and older adult population. The study found that the associations between different individual PUFAs and age-related muscle loss may differ, with high concentrations of n-6 PUFAs being correlated with less muscle mass loss, while extremely high concentrations of n-3 PUFAs being correlated with more muscle loss.