Major peanut allergens are quickly released from peanuts when seeds are hydrated under specific conditions

Allergens release from their biological source is a critical step in allergic sensitization. We sought to investigate in vitro the role of hydration at 1:10 w/v without stirring and 1:5 w/v with and without stirring on the release of major and minor allergens from peanut kernels. We hypothesized that hydration plays a pivotal role in peanut allergens release, affecting major allergens predominantly, and that peanut-water ratio and stirring influence allergen diffusion. We found that major peanut allergen Ara h 1 was quickly released during hydration leading to a decrease in its content in the seed particularly at hydration performed at 1:5 w/v with stirring. Ara h 2 remained more preserved in the hydrated seed, while Ara h 3 showed no content decrease despite its important release into the hydration water. Minor allergens Ara h 8 and Ara h 9 have lower abundance in peanut leading to a reduction of their content in the seed after their diffusion into the water during hydration. The results also demonstrated that a higher seed-to-water ratio (1:5 w/v) and stirring had a more pronounced impact on allergen release.

Automated summary

This study investigated the impact of hydration on the release of major and minor allergens from peanut kernels. The results showed that hydration played a crucial role in the release of peanut allergens, with major allergen Ara h 1 being quickly released and minor allergens Ara h 8 and Ara h 9 diffusing into the water. The ratio of peanut to water and stirring had a significant impact on allergen release.