期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 23, 页码 -出版社
MDPI
DOI: 10.3390/ijms242317097
关键词
multiple sclerosis; disease activity; transient receptor potential vanilloid 4; TRPV4; microglia; inflammation; experimental autoimmune encephalitis; EAE; cuprizone
This study investigates the role of TRPV4 in microglial activity and disease activity in a mouse model of multiple sclerosis (MS). The findings suggest that although TRPV4 modulates microglial activity, it does not impact disease activity.
Microglia are found pathologically at all stages of multiple sclerosis (MS) lesion development and are hypothesized to contribute to both inflammatory injury and neuroprotection in the MS brain. Transient receptor potential vanilloid 4 (TRPV4) channels are widely expressed, play an important role as environmental sensors, and are involved in calcium homeostasis for a variety of cells. TRPV4 modulates myeloid cell phagocytosis in the periphery and microglial motility in the central nervous system. We hypothesized that TRPV4 deletion would alter microglia phagocytosis in vitro and lessen disease activity and demyelination in experimental autoimmune encephalitis (EAE) and cuprizone-induced demyelination. We found that genetic deletion of TRPV4 led to increased microglial phagocytosis in vitro but did not alter the degree of demyelination or remyelination in the cuprizone mouse model of MS. We also found no difference in disease in EAE following global or microglia-specific deletion of Trpv4. Additionally, lesioned and normal appearing white matter from MS brains exhibited similar TRPV4 expression compared to healthy brain tissue. Taken together, these findings indicate that TRPV4 modulates microglial activity but does not impact disease activity in mouse models of MS, suggesting a muted and/or redundant role in MS pathogenesis.
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