Human Umbilical Cord Mesenchymal-Stem-Cell-Derived Extracellular Vesicles Reduce Skin Inflammation In Vitro

The protective roles of extracellular vesicles derived from human umbilical cord mesenchymal stem cells against oxazolone-induced damage in the immortalized human keratinocyte cell line HaCaT were investigated. The cells were pretreated with or without UCMSC-derived extracellular vesicles 24 h before oxazolone exposure. The pretreated UVMSC-EVs showed protective activity, elevating cell viability, reducing intracellular ROS, and reducing the changes in the mitochondrial membrane potential compared to the cells with a direct oxazolone treatment alone. The UCMSC-EVs exhibited anti-inflammatory activity via reducing the inflammatory cytokines IL-1 beta and TNF-alpha. A mechanism study showed that the UCMSC-EVs increased the protein expression levels of SIRT1 and P53 and reduced P65 protein expression. It was concluded that UVMSC-EVs can induce the antioxidant defense systems of HaCaT cells and that they may have potential as functional ingredients in anti-aging cosmetics for skin care.

Automated summary

The protective effects of extracellular vesicles derived from human umbilical cord mesenchymal stem cells (UCMSC-EVs) against oxazolone-induced damage in HaCaT cells were investigated. UCMSC-EVs pretreatment elevated cell viability, reduced intracellular ROS, and prevented changes in mitochondrial membrane potential. Furthermore, UCMSC-EVs exhibited anti-inflammatory activity by reducing the expression of inflammatory cytokines.