Effects of Chronic Heat Stress on Kidney Damage, Apoptosis, Inflammation, and Heat Shock Proteins of Siberian Sturgeon (Acipenser baerii)

Chronic heat stress caused by global warming can have serious implications for fish survival. The kidney plays a central role in many homeostatic functions, including water and electrolyte regulation. However, there is limited knowledge about the effect of heat stress on fish kidneys. In this study, water temperatures were increased from 20 degrees C to 24 degrees C and 28 degrees C in 8 days at a warming rate of 1 degrees C/d, and then maintained for 12 days. We investigated the effects of mild heat stress (24 degrees C) and high heat stress (28 degrees C) on Siberian Sturgeon (Acipenser baerii) kidneys using histological observation, flow cytometry detection, and RT-qPCR. Our histological observations revealed that heat stress caused significant infiltration of inflammatory cells in the kidney, especially at 28 degrees C. The flow cytometry assay demonstrated a significant increase in the number of apoptotic cells after heat stress at 28 degrees C compared to a control group at 20 degrees C (p = 0.033). The level of plasma creatinine was significantly increased in the 28 degrees C group compared to the control group (p = 0.001). In addition, the mRNA expression levels of heat shock protein GRP75 increased (p = 0.009). The results indicate that heat stress at 28 degrees C caused damage to the kidneys of A. baerii and triggered the protective response of heat shock proteins. In conclusion, this study contributes to the understanding of the coping strategies of the kidney of A. baerii for chronic heat stress.

Automated summary

Chronic heat stress caused by global warming has serious implications for fish survival, affecting the kidney with inflammatory cell infiltration, increased apoptotic cell numbers, elevated plasma creatinine levels, and increased expression of heat shock protein GRP75.