期刊
PARASITES & VECTORS
卷 16, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13071-023-06041-3
关键词
Cystic echinococcosis; NLRP3 inflammasome; KDM6B; Trimethylated histone H3 lysine 27
Our study reveals that EgCF inhibits the expression of KDM6B and H3K27me3 demethylation, leading to the transcriptional inhibition of NLRP3 and IL-1 beta. These findings provide new insights into the immune evasion mechanisms of E. granulosus.
Background Echinococcus granulosus can manipulate its host's immune response to ensure its own survival. However, the effect of histone modifications on the regulation of the NOD-like receptor protein 3 (NLRP3) inflammasome and downstream interleukin-1 beta (IL-1 beta) production in response to the parasite is not fully understood.Methods We evaluated IL-1 beta secretion through enzyme-linked immunosorbent assay and assessed reactive oxygen species levels using the dichlorodihydrofluorescein diacetate probe. Western blotting and quantitative real-time polymerase chain reaction were performed to examine the expression of NLRP3 and IL-1 beta in mouse peritoneal macrophages and Tohoku Hospital Pediatrics-1 cells, a human macrophage cell line. The presence of trimethylated histone H3 lysine 27 (H3K27me3) modification on NLRP3 and IL-1 beta promoters was studied by chromatin immunoprecipitation.Results Treatment with E. granulosus cyst fluid (EgCF) considerably reduced IL-1 beta secretion in mouse and human macrophages, although reactive oxygen species production increased. EgCF also suppressed the expression of NLRP3 and IL-1 beta. Mechanistically, EgCF prompted the enrichment of repressive H3K27me3 modification on the promoters of both NLRP3 and IL-1 beta in macrophages. Notably, the presence of EgCF led to a significant reduction in the expression of the H3K27me3 demethylase KDM6B.Conclusions Our study revealed that EgCF inhibits KDM6B expression and H3K27me3 demethylation, resulting in the transcriptional inhibition of NLRP3 and IL-1 beta. These results provide new insights into the immune evasion mechanisms of E. granulosus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据