Detection limit of FT-IR-based bacterial typing based on optimized sample preparation and typing model

Article Biochemistry & Molecular Biology

Protein FT-IR amide bands are beneficial to bacterial typing

Jinghang Xie et al.

Summary: This study investigated the influence of water on the bacterial protein amide bands using FT-IR spectroscopy. The results showed that the FT-IR signals of protein amide bands are beneficial for bacterial typing.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

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Article Engineering, Environmental

Molecular microbiological approaches reduce ambiguity about the sources of faecal pollution and identify microbial hazards within an urbanised coastal environment

Nathan L. R. Williams et al.

Summary: By using molecular microbiological approaches, the causes and point sources of faecal contamination at an urbanised beach have been precisely pinpointed, which helps identify the most suitable locations for targeted management of water quality at the beach.

WATER RESEARCH (2022)

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Article Microbiology

Use of Fourier-Transform Infrared Spectroscopy With IR Biotyper® System for Legionella pneumophila Serogroups Identification

Maria Rosaria Pascale et al.

Summary: Legionella pneumophila is a common bacteria causing severe pneumonia. Traditional methods for its identification are not accurate enough, while mass spectrometry and infrared spectroscopy have been proven to be rapid and effective. This study evaluated an infrared spectroscopy system and found an accuracy of 95.49%. This method is superior to traditional agglutination tests and can be used to trace the infection source and implement control strategies.

FRONTIERS IN MICROBIOLOGY (2022)

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Article Biochemical Research Methods

Using MetaboAnalyst 5.0 for LC-HRMS spectra processing, multi-omics integration and covariate adjustment of global metabolomics data

Zhiqiang Pang et al.

Summary: Liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) is widely used in global metabolomics studies, but there are still challenges in data processing, statistical analysis, and functional interpretation. To overcome these barriers, the MetaboAnalyst platform has been updated to provide step-by-step instructions.

NATURE PROTOCOLS (2022)

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Article Biochemical Research Methods

Comparison of FT-IR with whole-genome sequencing for identification of maternal-to-neonate transmission of antibiotic-resistant bacteria

Maya Azrad et al.

Summary: Using Fourier transform infrared spectrometry (FT-IR), this study confirmed the vertical transmission of antibiotic-resistant bacteria from mothers to neonates.

JOURNAL OF MICROBIOLOGICAL METHODS (2022)

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Review Chemistry, Analytical

Discrimination of bacteria using whole organism fingerprinting: the utility of modern physicochemical techniques for bacterial typing

Najla AlMasoud et al.

Summary: Rapid and accurate classification and discrimination of bacteria is crucial, especially with the rising concern of antimicrobial resistance. Modern analytical methods such as mass spectrometry and vibrational spectroscopy have emerged as essential alternative approaches, offering rapid, reliable, and cost-effective advantages for analysing various types of bacterial samples.

ANALYST (2021)

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Article Chemistry, Analytical