期刊
ARCHIVES OF MEDICAL RESEARCH
卷 55, 期 1, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.arcmed.2023.102909
关键词
Clinical biomarkers; Transcriptomics; Metabolomics; Multi-omics; microRNA; Gestational hypertension
Through integrating next-generation sequencing and metabolomics multi-omics analysis of placenta, this study identified differentially expressed miRNAs, their target genes, altered metabolites, and metabolic pathways in gestational hypertension (GH) patients. The findings obtained from this study may contribute to further understanding of the molecular pathways associated with GH and the evaluation of prognostic markers.
Background. Gestational hypertension (GH) is a severe complication that occurs after 20 weeks of pregnancy; however, its molecular mechanisms are not yet fully understood.Objective. Through this case-control discovery phase study, we aimed to find disease -specific candidate placental microRNAs (miRNAs) and metabolite markers for differentiating GH by integrating next-generation sequencing and metabolomics multi-omics analysis of placenta. Using small RNA sequencing and metabolomics of placental tis-sues of healthy pregnant (HP, n = 24) and GH subjects ( n = 20), the transcriptome and metabolome were characterized in both groups.Results. The study identified a total of 44 downregulated placental miRNAs which includes three novel, three mature and 38 precursor miRNAs. Six miRNAs includ-ing three mature (hsa-miR-181a-5p, hsa-miR-498-5p, and hsa-miR-26b-5p) and three novel (NC_000016.10_1061, NC_000005.10_475, and NC_000001.11_53) were considered for final target prediction and functional annotation. Integrative analysis of differentially expressed miRNAs and metabolites yielded five pathways such as purine, glutathione, glycerophospholipid, inositol phosphate and beta-alanine to be significantly perturbed in GH. We present fourteen genes (LPCAT1, LPCAT2, DGKH, PISD, GPAT2, PTEN, SACM1L, PGM2, AMPD3, AK7, AK3, CNDP1, IDH2, and ODC1) and eight metabolites (xanthosine, xanthine, spermine, glycine, CDP-Choline, glyceraldehyde 3 -phosphate, beta-alanine, and histidine) with potential to distinguish GH and HP.Conclusion. The differential expression of miRNAs, their target genes, altered metabolites and metabolic pathways in GH patients were identified for the first time in our study. Further, the altered miRNAs and metabolites were integrated to build their inter -connectivity network. The findings obtained from our study may be used as a valuable source to further unravel the molecular pathways associated with GH and also for the evaluation of prognostic markers.(c) 2023 Instituto Mexicano del Seguro Social (IMSS). Published by Elsevier Inc. All rights reserved.
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