4.7 Article

Free PCR virus detection via few-layer bismuthene and tetrahedral DNA nanostructured assemblies

期刊

TALANTA
卷 269, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.talanta.2023.125405

关键词

SARS-CoV-2; Few -layer bismuthene; Electrochemiluminescence; Tetrahedral DNA nanostructures; Biocatalysis; DNA biosensor

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In this study, a highly sensitive method based on biocatalyzed electrochemiluminescence approach was developed. The method utilizes few-layer bismuthene as a platform for immobilizing tetrahedral DNA nanostructures that are specifically designed to detect a specific gene sequence of SARS-CoV-2. The method exhibits a low limit of detection and a wide linear range, and can be used to directly detect the virus in samples from COVID-19 patients.
In this work we describe a highly sensitive method based on a biocatalyzed electrochemiluminescence approach. The system combines, for the first time, the use of few-layer bismuthene (FLB) as a platform for the oriented immobilization of tetrahedral DNA nanostructures (TDNs) specifically designed and synthetized to detect a specific SARS-CoV-2 gene sequence. In one of its vertices, these TDNs contain a DNA capture probe of the open reading frame 1 ab (ORF1ab) of the virus, available for the biorecognition of the target DNA/RNA. At the other three vertices, there are thiol groups that enable the stable anchoring/binding to the FLB surface. This novel geometry/approach enables not only the binding of the TDNs to surfaces, but also the orientation of the capture probe in a direction normal to the bismuthine surface so that it is readily accessible for binding/recognition of the specific SARS-CoV-2 sequence. The analytical signal is based on the anodic electrochemiluminescence (ECL) intensity of luminol which, in turn, arises as a result of the reaction with H2O2, generated by the enzymatic reaction of glucose oxidation, catalyzed by the biocatalytic label avidin-glucose oxidase conjugate (Av-GOx), which acts as co-reactant in the electrochemiluminescent reaction. The method exhibits a limit of detection (LOD) of 4.31 aM and a wide linear range from 14.4 aM to 1.00 mu M, and its applicability was confirmed by detecting SARS-CoV-2 in nasopharyngeal samples from COVID-19 patients without the need of any amplification process.

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