期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/ijms242216116
关键词
Campylobacter; poultry; fluoroquinolone-resistance; phylogeny; aac(6 ')-Ib-cr
This study reports the first identification of Campylobacter coli strains harboring both aac(6 ')-Ib and aac(6 ')-Ib-cr variants in Tunisia. These genes are present in isolates exhibiting resistance to multiple antibiotics.
The aac(6 ')-Ib gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant aac(6 ')-Ib-cr gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A total of 132 Campylobacter isolates, including 91 C. jejuni and 41 C. coli, were selected from broiler hens isolates. The aac(6 ')-Ib gene was amplified using PCR and was subsequently digested with the BtsCI restriction enzyme to identify aac(6 ')-Ib-cr. Among these isolates, 31 out of 41 C. coli (75.6%) and 1 (0.98%) C. jejuni were positive for the aac(6 ')-Ib gene, which was identified as the aac(6 ')-Ib-cr variant in 10 (32.25%) C. coli isolates. This variant was correlated with mutations in gyrA (Thr-86-Ile), as well as resistance to FQs. This study is the first report in Tunisia on Campylobacter coli strains harboring both the aac(6 ')-Ib and aac(6 ')-Ib-cr variants. These genes were present in Campylobacter isolates exhibiting resistance to multiple antibiotics, which restricts the range of available treatments.
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