期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/ijms242216345
关键词
intravital imaging; lipopolysaccharide; leukocytes; microcirculation; inflammation
Intravital microscopy (IVM) is a powerful imaging tool that captures real-time biological processes, allowing observation of complex cellular interactions in vivo. It has become essential in characterizing immune responses through visualization of changes in leukocyte-endothelial interactions and microcirculation.
Intravital microscopy (IVM) is a powerful imaging tool that captures biological processes in real-time. IVM facilitates the observation of complex cellular interactions in vivo, where ex vivo and in vitro experiments lack the physiological environment. IVM has been used in a multitude of studies under healthy and pathological conditions in different organ systems. IVM has become essential in the characterization of the immune response through visualization of leukocyte-endothelial interactions and subsequent changes within the microcirculation. Lipopolysaccharide (LPS), a common inflammatory trigger, has been used to induce inflammatory changes in various studies utilizing IVM. In this review, we provide an overview of IVM imaging of LPS-induced inflammation in different models, such as the brain, intestines, bladder, and lungs.
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